lewis-x-antigen has been researched along with Burkitt-Lymphoma* in 2 studies
2 other study(ies) available for lewis-x-antigen and Burkitt-Lymphoma
| Article | Year |
|---|---|
Frequent but nonrandom expression of myeloid markers on de novo childhood acute lymphoblastic leukemia.
The expression of the myeloid markers CD13, CD33, and CD15 in two hundred and eighty-three cases of de novo childhood acute lymphoblastic leukemia (ALL) is examined. The expression of at least one marker is a frequent event which is noted in 64% and 74% of B- and T-lineage ALL cases, respectively. Certain patterns of myeloid antigen expression can be recognized including: no expression of CD13, CD33, and CD15 in mature B-ALL, significantly higher levels of CD13 and CD33 and significantly lower levels of CD15 in TEL-AML1-positive B cell precursor ALL, no expression of CD13 and CD33 in E2A-PBX1-positive B cell precursor ALL cases and common T-ALL (double positive for CD4 and CD8), and no expression of CD13 in MLL-AF4-positive B cell precursor ALL cases. Although the numbers in some ALL subtypes are small, these patterns are consistent with nonrandom expression of myeloid markers in de novo childhood ALL. Topics: Antigens, CD; Antigens, Differentiation, Myelomonocytic; Biomarkers, Tumor; Burkitt Lymphoma; CD13 Antigens; Cell Differentiation; Child; Core Binding Factor Alpha 2 Subunit; Homeodomain Proteins; Humans; Lewis X Antigen; Myeloid Cells; Oncogene Proteins, Fusion; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Sialic Acid Binding Ig-like Lectin 3 | 2007 |
Distinct substrate specificities of five human alpha-1,3-fucosyltransferases for in vivo synthesis of the sialyl Lewis x and Lewis x epitopes.
Five different human alpha-1,3-fucosyltransferase genes, the Fuc-TIII, Fuc-TIV, Fuc-TV, Fuc-TVI and Fuc-TVII genes, have been cloned to date. We transfected HeLa cells and Namalwa cells with each of the five different genes, and established a series of stable cloned transformant cells. Thin-layer chromatography immunostaining analysis revealed that all five enzymes were able to synthesize sialyl Lewis x (sLe(x)) epitopes on glycolipids in HeLa cells, but each enzyme showed a different preference as to the carbohydrate chain length on glycolipids as acceptor substrates. Fuc-TIII and Fuc-TV showed very similar patterns of sLe(x) positive bands, which indicated that the enzymes have similar acceptor substrate specificities. Fuc-TVI exhibited a little different pattern from those of the former two enzymes. Fuc-TIV and Fuc-TVII showed similarity in the positive bands, however, their patterns were quite different from those of the former three enzymes. Four enzymes except for Fuc-TVII were able to synthesize the Lewis x (Le(x)) epitope on glycolipids in HeLa cells. Fuc-TV alone showed a little different pattern of Le(x) positive bands from those of the other three enzymes. Flow cytometric analysis of HeLa cells and Namalwa cells again demonstrated the similar specificities of Fuc-TIII and Fuc-TV. They exhibited similar stronger staining with FH6 (anti-sLe(x)) antibodies than that with the other enzymes. A phylogenetic tree of the five enzymes constructed using the neighbor-joining method showed good agreement with the similarities in the enzyme substrate specificity. Topics: Burkitt Lymphoma; Clone Cells; Flow Cytometry; Fucosyltransferases; HeLa Cells; Humans; Isoenzymes; Lewis X Antigen; Oligosaccharides; Recombinant Proteins; Sialyl Lewis X Antigen; Substrate Specificity; Transcription, Genetic; Transfection | 1997 |