levorphanol and Glioma

Neuroblastoma X glioma NG108-15 hybrid cells cultured in a chemically defined medium within 3 cell passages, exhibited viability, growth rate and morphology similar to those of cells grown in medium supplemented with 5% fetal calf serum. Hybrid cells cultured in the chemically defined medium within these periods of time also did not exhibit a difference in basal adenylate cyclase activity nor in the enzymatic activities stimulated by adenosine, forskolin, NaF, GppNHp or Mn2+. Furthermore, opiate receptor density in chemically defined medium cultured cells remained identical to that in cells cultured in 5% fetal calf serum. The acute and chronic effects of opiates on adenylate cyclase were similar for cells grown under either set of conditions.

Topics: Adenylyl Cyclases; Culture Media; Etorphine; Glioma; Hybrid Cells; Levorphanol; Morphinans; Neuroblastoma; Receptors, Opioid

Topics: Animals; Cyclic AMP; Cyclic GMP; Dextrorphan; Dose-Response Relationship, Drug; Glioma; Hybrid Cells; Levorphanol; Mice; Morphine; Naloxone; Neuroblastoma; Neuroglia; Neurons; Rats; Receptors, Drug