leukotriene-e4 and Ischemic-Attack--Transient

We investigated the hypothesis that cerebral prostanoid and peptidoleukotriene (LTs) (LTC4/D4/E4/F4) synthesis are increased during postischemic reperfusion of newborn pig brains. Prostanoids and LTs extracted from brain tissue were determined by RIA in sham-control piglets and at 1h, 3h, or 12h after a 20-min period of total cerebral ischemia. During reperfusion following ischemia, all regional brain tissue (cerebrum, brain stem and cerebellum) prostanoids (6-keto-PGF1 alpha, TXB2, PGE2 and PGF2 alpha) were increased at 1h compared with those in sham-control piglets. Only cerebral and brain stem 6-keto-PGF1 alpha and cerebral TXB2 remained elevated at 3h postischemia and all prostanoids returned to control levels by 12h postischemia. Brain tissue LTs were lower than prostanoids and were not altered 1, 3, or 12h following ischemia. These data indicate that 1) newborn pig brain tissue prostanoids are increased initially, and then returned to control levels at later stages of reperfusion following ischemia; 2) LTs are present in newborn pig brain tissue, but are not increased by ischemia/reperfusion injury and therefore probably do not play a significant role in cerebral ischemia-reperfusion injury.

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Animals, Newborn; Brain; Brain Stem; Cerebellum; Dinoprost; Dinoprostone; Female; Ischemic Attack, Transient; Leukotriene E4; Male; Prostaglandins; Reperfusion; SRS-A; Swine; Thromboxane B2

Five minutes after the onset of seizures high amounts of immunoreactive prostaglandin (PG) F2 alpha and smaller amounts of sulphidopeptide (SP)-leukotriene (LT)-like immunoreactivity could be detected in gerbil brain tissue. Bilateral carotid artery ligation followed by 15 min of reperfusion even more enhanced brain tissue contents of PGF2 alpha and SP-LT-like material. Analysis of the immunoreactive SP-LT-like material by reversed phase high pressure liquid chromatography (h.p.l.c.) revealed immunoreactivity co-eluting with authentic LTC4 and LTD4.

Topics: Animals; Chromatography, High Pressure Liquid; Gerbillinae; Ischemic Attack, Transient; Leukotriene E4; Seizures; SRS-A