leukotriene-d4 and Glioma

leukotriene-d4 has been researched along with Glioma* in 2 studies

Other Studies

2 other study(ies) available for leukotriene-d4 and Glioma

Article	Year
[Effects of agonist and antagonist of cysteinyl leukotriene receptors on differentiation of rat glioma C6 cells]. Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2011, Volume: 40, Issue:2 To investigate the role of cysteinyl leukotriene (CysLT) receptors in the differentiation of rat glioma C6 cells.. Rat glioma C6 cells were treated with the agonist LTD(4), the CysLT(1) receptor antagonist montelukast and the differentiation inducer forskolin. Cell morphology and GFAP protein expression were determined after treatments.. Forskolin (10 μmol/L) induced morphological changes and GFAP protein expression (cell differentiation) in C6 cells, but LTD(4) (0.1-100 nmol/L) did not induce these changes. Montelukast (1 μmol/L) alone did not affect C6 cell differentiation, while it induced the differentiation when combined with the LTD(4) (100 nmol/L).. The CysLT(2) receptor may modulate the differentiation of rat glioma C6 cells. Topics: Acetates; Animals; Cell Differentiation; Cell Line, Tumor; Colforsin; Cyclopropanes; Cysteine; Glioma; Leukotriene Antagonists; Leukotriene D4; Leukotrienes; Quinolines; Rats; Receptors, Leukotriene; Sulfides	2011
[Method for screening cysteinyl leukotriene receptor 2 antagonists and preliminary screening of compounds]. Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2009, Volume: 38, Issue:6 To establish a method for screening cysteinyl leukotriene receptor 2 (CysLT(2)) antagonists and to preliminarily screen a series of synthetic compounds.. Rat glioma cell line (C6 cells) highly expressing CysLT(2) receptor was used. Intracellular calcium concentration was measured after stimulation with the agonist LTD(4),which was used to screen compounds with antagonist activity for CysLT(2) receptor. Bay u9773, a CysLT1/CysLT(2) receptor non-selective antagonist, and AP-100984, a CysLT(2) receptor antagonist, were used as control.. PT-PCR showed a higher expression of CysLT(2) receptor in C6 cells. LTD(4) at 1 mumol/L significantly increased intracellular calcium in C6 cells; the maximal effect was about 37.5% of ATP, a positive stimulus.LTD(4)-induced increase of intracellular calcium was blocked by CysLT(2) receptor antagonists, but not by CysLT(1) receptor antagonists. Among the synthetic compounds, D(XW-)1,2,13,23,29 and 30 inhibited LTD(4)-induced increase of intracellular calcium.. LTD(4)-induced change in intracellular calcium in C6 cells can be used as a screening method for CysLT(2) receptor antagonists. The compounds, D(XW-)1,2,13,23,29 and 30, possess antagonist activity for CysLT(2) receptor. Topics: Animals; Brain Neoplasms; Cell Line, Tumor; Drug Evaluation, Preclinical; Glioma; Leukotriene Antagonists; Leukotriene D4; Rats; Receptors, Leukotriene	2009

Article

Year

[Effects of agonist and antagonist of cysteinyl leukotriene receptors on differentiation of rat glioma C6 cells].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2011, Volume: 40, Issue:2

To investigate the role of cysteinyl leukotriene (CysLT) receptors in the differentiation of rat glioma C6 cells.. Rat glioma C6 cells were treated with the agonist LTD(4), the CysLT(1) receptor antagonist montelukast and the differentiation inducer forskolin. Cell morphology and GFAP protein expression were determined after treatments.. Forskolin (10 μmol/L) induced morphological changes and GFAP protein expression (cell differentiation) in C6 cells, but LTD(4) (0.1-100 nmol/L) did not induce these changes. Montelukast (1 μmol/L) alone did not affect C6 cell differentiation, while it induced the differentiation when combined with the LTD(4) (100 nmol/L).. The CysLT(2) receptor may modulate the differentiation of rat glioma C6 cells.

Topics: Acetates; Animals; Cell Differentiation; Cell Line, Tumor; Colforsin; Cyclopropanes; Cysteine; Glioma; Leukotriene Antagonists; Leukotriene D4; Leukotrienes; Quinolines; Rats; Receptors, Leukotriene; Sulfides

2011

[Method for screening cysteinyl leukotriene receptor 2 antagonists and preliminary screening of compounds].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2009, Volume: 38, Issue:6

To establish a method for screening cysteinyl leukotriene receptor 2 (CysLT(2)) antagonists and to preliminarily screen a series of synthetic compounds.. Rat glioma cell line (C6 cells) highly expressing CysLT(2) receptor was used. Intracellular calcium concentration was measured after stimulation with the agonist LTD(4),which was used to screen compounds with antagonist activity for CysLT(2) receptor. Bay u9773, a CysLT1/CysLT(2) receptor non-selective antagonist, and AP-100984, a CysLT(2) receptor antagonist, were used as control.. PT-PCR showed a higher expression of CysLT(2) receptor in C6 cells. LTD(4) at 1 mumol/L significantly increased intracellular calcium in C6 cells; the maximal effect was about 37.5% of ATP, a positive stimulus.LTD(4)-induced increase of intracellular calcium was blocked by CysLT(2) receptor antagonists, but not by CysLT(1) receptor antagonists. Among the synthetic compounds, D(XW-)1,2,13,23,29 and 30 inhibited LTD(4)-induced increase of intracellular calcium.. LTD(4)-induced change in intracellular calcium in C6 cells can be used as a screening method for CysLT(2) receptor antagonists. The compounds, D(XW-)1,2,13,23,29 and 30, possess antagonist activity for CysLT(2) receptor.

Topics: Animals; Brain Neoplasms; Cell Line, Tumor; Drug Evaluation, Preclinical; Glioma; Leukotriene Antagonists; Leukotriene D4; Rats; Receptors, Leukotriene

2009