leukotriene-c4 and Glomerulonephritis--IGA

IgA nephropathy is diagnosed by renal biopsy, an invasive procedure with a risk of significant complications. Noninvasive approaches are needed for possible diagnostic purposes and especially for monitoring disease activity or responses to treatment. In this pilot project, we assessed the utility of urine samples as source of biomarkers of IgA nephropathy. We used spot urine specimens from 19 healthy controls, 11 patients with IgA nephropathy, and 8 renal-disease controls collected on day of renal biopsy. Urine samples were analyzed using untargeted metabolomic and targeted proteomic analyses by several experimental techniques: liquid chromatography coupled with mass spectrometry, immunomagnetic isolation of target proteins coupled with quantitation by mass spectrometry, and protein arrays. No single individual biomarker completely differentiated the three groups. Therefore, we tested the utility of several markers combined in a panel. Discriminant analysis revealed that combination of seven markers, three metabolites (dodecanal, 8-hydroxyguanosine, and leukotriene C

Topics: Adult; Aged; Aldehydes; alpha 1-Antitrypsin; Biomarkers; Case-Control Studies; Female; Glomerulonephritis, IGA; Guanosine; Heparitin Sulfate; Humans; Immunoglobulin A; Leukotriene C4; Male; Metabolome; Middle Aged; Proteome; Uromodulin