leukotriene-b4 and Periodontal-Pocket

Inflammatory and anti-inflammatory mediators may play a significant role in patients with gingivitis. The purpose of this study was to assess the short-term effects of the systemic administration of two different concentrations of aspirin (81 and 325 mg/day, by mouth) on clinical periodontal parameters and gingival crevicular fluid (GCF) levels of 15-epi-lipoxin A4 (15-epi-LXA4), lipoxin A4, leukotriene B4 (LTB4), prostaglandin E2 (PGE2), and interleukin (IL)-6 and -1beta in a sample of naturally occurring gingivitis patients.. At day 0, after initial screening for entry, baseline periodontal parameters, including bleeding on probing (BOP), periodontal probing depths (PDs), and plaque index (PI) were measured, and GCF was sampled from 12 intrasulcular sites with filter paper strips for the measurement of six types of inflammatory and anti-inflammatory mediators using competitive enzyme immunoassay and enzyme-linked immunosorbent assay (prevalues). Forty-seven subjects were assigned randomly to one of three treatment groups: placebo (15 subjects); aspirin, 81 mg (16 subjects); and aspirin, 325 mg (16 subjects) once daily. On day 7, subjects were recalled for the measurement of periodontal parameters and collection of GCF samples for the measurement of six types of mediators (postvalues).. Changes in inflammatory and anti-inflammatory mediator levels were not statistically significant for any of the three treatment groups. However, when pre- and postvalues were compared in the subjects receiving aspirin, 325 mg, there was a negative trend in the relationship between 15-epi-LXA4 and PGE2, whereas the relationship between LTB4 and PGE2 was not as strong. This might indicate that the subjects responding to aspirin-mediated PGE2 suppression effects produced higher 15-epi-LXA4 in GCF than non-responders. No statistically significant differences in PD and PI between pre- and postvalues were found for any of the three treatment groups. However, the results demonstrated a significant increase in BOP when aspirin, 325 mg was compared to placebo (P <0.001) and aspirin, 81 mg (P = 0.001).. Aspirin can have an affect on BOP in naturally occurring gingivitis patients. Although most of the inflammatory mediators did not show significantly detectable changes after aspirin treatment for 7 days, the trend of aspirin-associated increases of 15-epi-LXA4 implied that this recently discovered aspirin-dependent eicosanoid may be associated with the increased incidence of BOP observed in the subjects who received aspirin therapy.

Topics: Adult; Anti-Inflammatory Agents, Non-Steroidal; Aspirin; Dental Plaque Index; Dinoprostone; Female; Gingival Crevicular Fluid; Gingival Hemorrhage; Gingivitis; Humans; Inflammation Mediators; Interleukin-1beta; Interleukin-6; Leukotriene B4; Lipoxins; Male; Periodontal Pocket; Placebos

Here we determine the salivary levels of leukotriene B4 (LTB4 ) and its relation with salivary mucin and alveolar bone level.. LTB4 is a membrane-derived lipid mediator formed from arachidonic acid. It is among the most potent stimulants of polymorphonuclear leukocytes providing the first host defense against infections. Leukotrienes also induce bone resorption. Because LTB4 is present in the oral cavity the aim of the present study was to explore the role of LTB4 in patients with periodontal disease.. Eighty-one subjects were clinically examined and distributed into four groups, namely, clinically healthy, mild, moderate and severe periodontitis, according to periodontal status, classified into values of clinical attachment level and probing pocket depth. Unstimulated saliva was collected for 5 min. Salivary LTB4 was determined by an immune assay method, mucin was determined by a colorimetric method and radiographic assessment used to determine alveolar bone level.. Patients with mild periodontitis showed a decrease in salivary LTB4 levels while patients with severe periodontitis showed increased LTB4 levels. A significant positive correlation was observed between salivary LTB4 and clinical attachment level, salivary mucin concentration or alveolar bone level.. The close relation between salivary LTB4 and mucin levels suggested that LTB4 might be involved in the defense mechanism of the oral cavity. The correlation of LTB4 with the alveolar bone level indicates that they are one of the mediators responsible for bone resorption.

Topics: Adult; Alveolar Bone Loss; Alveolar Process; Colorimetry; Female; Humans; Leukotriene B4; Male; Middle Aged; Mucins; Neutrophils; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Periodontitis; Periodontium; Radiography, Bitewing; Saliva; Salivary Proteins and Peptides

The arachidonic acid metabolites prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) are inflammatory mediators which are likely to be involved in the pathogenesis of periodontal disease. PGE2 mediates vasodilatation, increases vascular permeability, enhances pain perception by bradykinin and histamine, alters connective tissue metabolism and enhances osteoclastic bone resorption. LTB4 causes the accumulation of inflammatory cells in the inflamed sites, and degranulation of polymorphonuclear leukocytes.. To measure gingival crevicular fluid (GCF) levels of PGE2, LTB4 and periodontal health.. The periodontal condition of 24 subjects was evaluated on the basis of plaque index, gingival index, probing depth, and attachment level. GCF samples were collected from one or two site(s) of each sextant per subject and the volume was measured using Periotron 6000. Samples were then assayed for PGE2 and LTB4 using a competitive enzyme immunoassay. Mean PGE2 and LTB4 levels were determined for each subject and group means compared.. Significant differences in the levels of PGE2 and LTB4 were found between patients with periodontitis, and non-periodontitis individuals (P < 0.001). The PGE2/LTB4 levels were positively correlated with the clinical parameters (P < 0.01) and reduced markedly after phase 1 of the periodontal treatment (P < 0.01). The total amount and concentration (ng ml-1) of LTB4 was positively correlated with the gingival index (P < 0.01).. These results indicate that the levels of PGE2 correlated with the severity of the periodontal status, and the levels of LTB4 correlated with gingival inflammation. Thus, our data suggest that the total amounts of PGE2/LTB4 may be good indicators for periodontal inflammation.

Topics: Adult; Alveolar Bone Loss; Dental Plaque; Dental Plaque Index; Dental Scaling; Dinoprostone; Female; Gingival Crevicular Fluid; Gingivitis; Humans; Immunoenzyme Techniques; Inflammation Mediators; Leukotriene B4; Male; Middle Aged; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Periodontitis; Periodontium; Regression Analysis; Root Planing; Subgingival Curettage