leukotriene-b4 and Parakeratosis

Vascular changes, both endothelial and functional, are crucial events in inflammatory responses.. To investigate the dynamics of endothelial cell (EC) and functional changes during acute inflammation in an in vivo model of the skin using leukotriene B4.. EC proliferation, vascular network size, vessel diameter (VD), and hypoxia-inducible factor (HIF)-1α were studied by immunohistochemical CD31/Ki67 double staining and single staining of HIF-1α. Cutaneous perfusion (CP) was assessed using the Twente Optical Perfusion Camera.. The initial phase illustrated an increase in VD, Ki67+ EC, and HIF-1α expression and late-phase vascular expansion. The HIF-1α and Ki67+ EC expression was limited. CP and VD were augmented after 24 h.. The early phase of inflammation is characterized by EC proliferation and HIF-1α expression. Vascular expansion continues over time. CP and VD are seen in both phases of inflammation. Angiogenesis, vascular network formation, and perfusion are time-dependent processes which are mutually related during inflammation.

Topics: Administration, Cutaneous; Adult; Cell Proliferation; Endothelial Cells; Endothelium; Female; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Inflammation; Laser-Doppler Flowmetry; Leukotriene B4; Male; Middle Aged; Neovascularization, Physiologic; Parakeratosis; Skin; Young Adult