leukotriene-b4 has been researched along with HTLV-I-Infections* in 2 studies
2 other study(ies) available for leukotriene-b4 and HTLV-I-Infections
| Article | Year |
|---|---|
HTLV-1-induced leukotriene B4 secretion by T cells promotes T cell recruitment and virus propagation.
The human T-lymphotropic virus type 1 (HTLV-1) is efficiently transmitted through cellular contacts. While the molecular mechanisms of viral cell-to-cell propagation have been extensively studied in vitro, those facilitating the encounter between infected and target cells remain unknown. In this study, we demonstrate that HTLV-1-infected CD4 T cells secrete a potent chemoattractant, leukotriene B4 (LTB4). LTB4 secretion is dependent on Tax-induced transactivation of the pla2g4c gene, which encodes the cytosolic phospholipase A2 gamma. Inhibition of LTB4 secretion or LTB4 receptor knockdown on target cells reduces T-cell recruitment, cellular contact formation and virus propagation in vitro. Finally, blocking the synthesis of LTB4 in a humanized mouse model of HTLV-1 infection significantly reduces proviral load. This results from a decrease in the number of infected clones while their expansion is not impaired. This study shows the critical role of LTB4 secretion in HTLV-1 transmission both in vitro and in vivo. Topics: Animals; CD4-Positive T-Lymphocytes; Cyclic AMP Response Element-Binding Protein; Female; Gene Products, tax; Group IV Phospholipases A2; Host-Pathogen Interactions; HTLV-I Infections; Human T-lymphotropic virus 1; Humans; Indoles; Infant, Newborn; Jurkat Cells; Leukotriene B4; Lipoxygenase Inhibitors; Male; Mice, Mutant Strains; NF-kappa B | 2017 |
Immunological signature of the different clinical stages of the HTLV-1 infection: establishing serum biomarkers for HTLV-1-associated disease morbidity.
This study aimed at establishing the immunological signature and an algorithm for clinical management of the different clinical stages of the HTLV-1-infection based on serum biomarkers. A panel of serum biomarkers was evaluated by four sets of innovative/non-conventional data analysis approaches in samples from 87 HTLV-1 patients: asymptomatic carriers (AC), putative HTLV-1 associated myelopathy/tropical spastic paraparesis (pHAM/TSP) and HAM/TSP. The analysis of cumulative curves and molecular signatures pointed out that HAM/TSP presented a pro-inflammatory profile mediated by CXCL10/LTB-4/IL-6/TNF-α/IFN-γ, counterbalanced by IL-4/IL-10. The analysis of biomarker networks showed that AC presented a strongly intertwined pro-inflammatory/regulatory net with IL-4/IL-10 playing a central role, while HAM/TSP exhibited overall immune response toward a predominant pro-inflammatory profile. At last, the classification and regression trees proposed for clinical practice allowed for the construction of an algorithm to discriminate AC, pHAM and HAM/TSP patients with the elected biomarkers: IFN-γ, TNF-α, IL-10, IL-6, IL-4 and CysLT. These findings reveal a complex interaction among chemokine/leukotriene/cytokine in HTLV-1 infection and suggest the use of the selected but combined biomarkers for the follow-up/diagnosis of disease morbidity of HTLV-1-infected individuals. Topics: Adult; Aged; Biomarkers; Blotting, Western; Chemokine CXCL10; Enzyme-Linked Immunosorbent Assay; Female; Host-Pathogen Interactions; HTLV-I Infections; Human T-lymphotropic virus 1; Humans; Inflammation Mediators; Interferon-gamma; Interleukin-10; Interleukin-4; Interleukin-6; Leukotriene B4; Male; Middle Aged; Paraparesis, Tropical Spastic; Receptors, Leukotriene; Signal Transduction; Tumor Necrosis Factor-alpha; Young Adult | 2015 |