leptin and Graves-Ophthalmopathy

To investigate the effects of rh-leptin on the proliferation, migration of orbital preadipocytes of thyroid associated ophthalmopathy (TAO).. Preadipocytes were cultured from orbital tissues of TAO patients after orbital decompression. The subcultured preadipocytes were incubated with different concentrations of rh-leptin. The proliferation of preadipocytes was tested by methyl thiazlyl tetrazolium (MTT) assay, and the migration was evaluated by crutch method.. With the treatment of 500 mg/L rh-leptin, the preadipocytes proliferated more than control (P<0.05), while 10-100 mg/L rh-leptin has no such significant effects. The migration of the preadipocytes was enhanced by rh-leptin, which showed statistic difference in 50-500 mg/L rh-leptin groups when compared with control group (P<0.05).. Rh-leptin could improve the proliferation and migration of TAO orbital preadipocytes in vitro, which suggests that leptin may play an important role in the pathogenesis of TAO.

Topics: Adipocytes; Cell Movement; Cell Proliferation; Cells, Cultured; Graves Ophthalmopathy; Humans; Leptin; Orbit; Recombinant Proteins