leptin and Aggressive-Periodontitis

Increasing evidence supports an association between periodontitis and systemic diseases. Leptin is involved both in the energy metabolism and inflammatory processes and is suggested to be a link between periodontal infection and systemic health. The present study aimed to evaluate the peripheral leptin concentration in patients with aggressive periodontitis (AgP) and to explore the relationship between leptin and systemic inflammation.. Ninety patients with AgP visiting the Clinic of the Periodontology Department, Peking University School and Hospital of Stomatology between July 2001 and May 2006, and 44 healthy controls (staff and student volunteers in the same institute) were recruited. Plasma levels of leptin and inflammatory cytokines including interleukin (IL)-1β, IL-6, tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) were measured by enzyme-linked immunosorbent assay. Correlation and multiple linear regression analysis were performed to analyze the association between plasma leptin level and other variables.. Plasma leptin level of AgP group was significantly higher than that of the control group (19.7 ± 4.4 ng/ml vs. 7.5 ± 1.3 ng/ml, P < 0.01). After controlling for age, gender, and body mass index, positive correlation was observed between plasma leptin concentration and log-transformed levels of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α and CRP), and the partial correlation coefficients ranged from 0.199 to 0.376 (P < 0.05). Log-transformed IL-1β and IL-6 levels entered the final regression model (standardized β were 0.422 and 0.461 respectively, P < 0.01).. Elevated plasma leptin concentration may be associated with increased systemic levels of inflammatory markers in AgP patients.

Topics: Adolescent; Adult; Aggressive Periodontitis; C-Reactive Protein; Enzyme-Linked Immunosorbent Assay; Female; Humans; Interleukin-6; Leptin; Male; Middle Aged; Tumor Necrosis Factor-alpha; Young Adult

To determine the level of leptin in gingival crevicular fluid (GCF) of patients with aggressive periodontitis (AgP) and to analyze the relationship between leptin and periodontal cilinical parameters.. Fifty-four patients with AgP and 30 healthy controls were recruited. Detailed clinical examinations were conducted, and clinical parameters such as bleeding index (BI), probing depth (PD), attachment loss (AL) were recorded. Two teeth were selected as test teeth in each subject, one from posterior area and the other from anterior region. The level of GCF leptin was measured by enzyme linked immunosorbent assay.. The level of GCF leptin in AgP patients was significantly lower than that of control subjects [(16.5±4.6) ng vs.(26.0±6.0) ng, P < 0.05]. The level of GCF leptin was negatively related to BI (-0.306, P < 0.01), PD (-0.346, P < 0.01) and AL (-0.250, P < 0.01).. AgP patients have significantly decreased level of GCF leptin and the level of leptin was negatively related to BI, PD and AL.

Topics: Adult; Aggressive Periodontitis; Case-Control Studies; Enzyme-Linked Immunosorbent Assay; Female; Gingival Crevicular Fluid; Humans; Leptin; Male; Periodontal Index

To detect the plasma leptin levels in patients with aggressive periodontitis (AgP) and to analyze the relationship between circulating leptin level and periodontal condition.. A total of 97 patients with AgP and 44 healthy controls were recruited. Detailed clinical examinations were conducted and clinical parameters such as bleeding index (BI), probing depth (PD), attachment loss (AL) were recorded. Plasma leptin level was measured by enzyme-linked immunosorbent assay.. The plasma leptin level in AgP group was significantly higher than that of control subjects [(20.0 ± 4.3) µg/L vs. (7.5 ± 1.3) µg/L, P < 0.01)]. The plasma leptin level was positively related to BI, PD and AL, and the r values were 0.647, 0.596 and 0.632 respectively (P < 0.01).. Plasma leptin concentration in AgP patients was significantly elevated compared with healthy controls. Circulating leptin level was positively related to periodontal parameters including BI, PD and AL.

Topics: Adolescent; Adult; Aggressive Periodontitis; Case-Control Studies; Female; Humans; Leptin; Male; Periodontal Attachment Loss; Periodontal Index; Plasma; Young Adult

The presence of leptin (OB) and soluble OB receptor (s-OB-R) in gingival tissue extract and gingival crevicular fluid has led the studies investigating the relationship between OB and periodontal diseases. This study aims to investigate the levels of OB and s-OB-R in serum and their presence in gingiva of healthy controls (HC), gingivitis (G), aggressive periodontitis (AP), and chronic periodontitis (CP) patients; and whether correlations exist between clinical and serum parameters, OB and s-OB-R. Seventy-seven subjects [HC (n = 20), G (n = 20), CP (n = 21), and AP (n = 16)] were included in this study. After the clinical periodontal parameter recordings and venous blood sampling, gingival tissues obtained. Serum parameters' levels determined with enzyme linked immune sorbent assay; and OB and OB-R in gingiva immunohistochemically. No significant differences were observed regarding the serum parameters [high sensitivity C-reactive protein (hs-CRP), lipids, OB, and s-OB-R] when the groups were compared (P > 0.0125). The serum OB has positive correlations with hs-CRP in the G group (P < 0.05), and s-OB-R has presented significant negative correlations with BOP in HC group (P < 0.05), with hs-CRP in G (P < 0.05) and AP groups (P < 0.05). The positive correlations were observed between the serum OB and HDL and body mass index in the CP group (P < 0.05). In all of the tissue samples of all groups, there was positive OB and OB-R immunoreactivity in the gingival epithelium. The gingival tissues contain both OB and OB-R. The serum levels of OB and s-OB-R do not vary between patients and with different periodontal conditions.

Topics: Adult; Aggressive Periodontitis; Body Mass Index; C-Reactive Protein; Cholesterol; Cholesterol, HDL; Cholesterol, LDL; Cholesterol, VLDL; Chronic Periodontitis; Dental Plaque Index; Epithelium; Female; Gingiva; Gingival Hemorrhage; Gingivitis; Humans; Leptin; Male; Middle Aged; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Receptors, Leptin; Triglycerides