laxaphycin-b and Ovarian-Neoplasms

Two laxaphycin type-B cyclic dodecapeptides, laxaphycins B5 and B6, were obtained from UIC 10484, a freshwater cf. Phormidium sp. Analysis using the 16S rRNA sequence found UIC 10484 to clade with UIC 10045, a known laxaphycin type-A and -B producer, and MS/MS analysis revealed the presence of two novel laxaphycin type-B compounds. The structures of the metabolites were elucidated using 2D NMR and MS/MS. The absolute configurations of the amino acids were determined by advanced Marfey's analysis. Both metabolites were evaluated against the same three cancer cell lines. The IC

Topics: Amino Acids; Breast Neoplasms; Cell Line, Tumor; Cyanobacteria; Female; Fresh Water; Humans; Magnetic Resonance Spectroscopy; Melanoma; Ovarian Neoplasms; Peptides, Cyclic; RNA, Ribosomal, 16S; Tandem Mass Spectrometry