landomycin-e has been researched along with Breast-Neoplasms* in 1 studies
1 other study(ies) available for landomycin-e and Breast-Neoplasms
| Article | Year |
|---|---|
Effect of landomycin E on expression of mRNA coding for transforming growth factor beta ligands and specific receptors in MCF-7 cells.
The aim of the present study was to investigate regulation of mRNA, coding for TGFbeta ligands and specific receptors, by novel antitumor antibiotic landomycin E (LE) in human breast adenocarcinoma cells of MCF-7 line, sensitive and resistant to anti-cancer agent doxorubicin.. Semi-quantitative analysis of mRNA expression was estimated using RT-PCR and DNA gel-electrophoresis methods. For comparison, another anti-cancer drug doxorubicin (adriamycin) was utilized. THE RESULTS obtained indicate that LE induces up-regulation of mRNA coding for TGFbeta receptors type I and type II in MCF-7 cells, as well as in their doxorubicin-resistant sub-line.. Anti-tumor action of LE may be mediated by TGFbeta, and a signaling pathway of this cytokine is not affected by the development of tumor cell resistance to doxorubicin. Topics: Adenocarcinoma; Aminoglycosides; Antineoplastic Agents; Breast Neoplasms; Cell Line, Tumor; Doxorubicin; Electrophoresis, Gel, Two-Dimensional; Female; Gene Expression; Humans; Ligands; Receptors, Transforming Growth Factor beta; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Transforming Growth Factor beta | 2005 |