laminaran and Gram-Positive-Bacterial-Infections

Pattern recognition receptors (PRRs) are biosensor proteins that bind to non-self pathogen associated molecular patterns (PAMPs). β-1,3-glucan recognition proteins (βGRPs) play an essential role in immune recognition and signaling pathway of insect innate immunity. Here, we report the cloning and characterization of cDNA of OfβGRP3 from Ostrinia furnacalis larvae. The OfβGRP3 contains 1455 bp open reading frame, encoding a predicted 484 amino acid residue protein. In hemocytes, the expression levels of OfβGRP3 in Escherichia coli-challenged group were higher than those of Bacillus subtilis-challenged group at 2, 4, 8, 10 and 12 h post injection (HPI). In fat body, OfβGRP3 expression in both B. subtilis and E. coli-challenged group was significantly higher than that in untreated group from 4 to 10 HPI, and then the expression continuously dropped from 12 to 36 HPI. The OfβGRP3 expression in laminarin-injected group was higher than that in lipopolysaccharides (LPS)-injected group in various test tissues from 4 to 24 HPI. The LT

Topics: Animals; Bacillus subtilis; beta-Glucans; Cells, Cultured; Cloning, Molecular; Gram-Positive Bacterial Infections; Guanine Nucleotide Exchange Factors; Immunity, Innate; Insect Proteins; Larva; Lipopolysaccharides; Moths; Pathogen-Associated Molecular Pattern Molecules; Receptors, Pattern Recognition; Signal Transduction