laidlomycin and Cell-Transformation--Viral

We have studied the effect of two carboxylic ionophores, monensin and laidlomycin, on the replication of measles virus in KB cells. The yield of infectious virus was markedly depressed at the concentrations of the ionophores which had no effect on overall viral protein synthesis. The ionophores selectively blocked the migration of hemagglutinin (H) glycoprotein from Golgi apparatus to the cell surface. As a result, H glycoprotein is prevented from being converted from incompletely glycosylated form to the mature form. The inhibitory effect on the transport and glycosylation of H was reversed, although gradually, upon the removal of the ionophores.

Topics: Anti-Bacterial Agents; Cell Transformation, Viral; DNA Replication; Fluorescent Antibody Technique; Furans; Hemagglutinins, Viral; Humans; KB Cells; Kinetics; Measles virus; Monensin; Virus Replication