lactoferrin and Multiple-Myeloma

In order to further evaluate the effects of rGM-CSF on the reconstituting granulopoiesis, plasma and serum levels of myeloperoxidase (MPO) and lactoferrin (LF), as well as serum levels of eosinophil cationic protein (ECP), were monitored daily during a period of 3-4 weeks following ABMT in a group of 22 patients treated with either rGM-CSF (n = 11) or placebo (n = 11). Despite faster increase in the neutrophil counts in the rGM-CSF group, we did not observe any difference either in P-MPO or in P-LF during the period of early engraftment (days 11-19). This finding indicates that the proliferative effect of rGM-CSF on the neutropoiesis may be overestimated when neutrophil counts alone are taken into consideration, and suggests that other mechanisms may have contributed to the increase in the number of circulating neutrophils. The ratio of the serum to plasma level of LF, but not of MPO, was higher in the rGM-CSF group, probably reflecting a specific in vivo neutrophil priming effect. In the rGM-CSF group there was a clear increase of S-ECP during the second and third week post transplant, corresponding to an increase in eosinophil counts, which indicates that rGM-CSF stimulated eosinophil reconstitution without causing excessive activation of the mature eosinophils.

Topics: Adolescent; Adult; Blood Proteins; Bone Marrow Transplantation; Double-Blind Method; Eosinophil Granule Proteins; Eosinophils; Granulocyte-Macrophage Colony-Stimulating Factor; Hematopoiesis; Humans; Lactoferrin; Leukocyte Count; Lymphoma; Middle Aged; Multiple Myeloma; Neutrophils; Peroxidase; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Recombinant Proteins; Ribonucleases

One explorative observational study in two parts was performed to examine early salivary changes in relation to oral mucositis (OM) in multiple myeloma patients treated with high-dose melphalan and autologous haematopoietic stem cell transplantation (HSCT). As cryotherapy was introduced after part A as regular care, its effect on OM could be evaluated.. Unstimulated whole-mouth saliva (UWS) and stimulated whole-mouth saliva (SWS) were collected, and OM was scored with the Oral Mucositis Nursing Instrument (OMNI) at days -3, 0, 4, 7, 11 and 14 after HSCT. Salivary flow rate, total protein (BCA), mucin 5B, albumin (western blot), total IgA, lactoferrin and myeloperoxidase levels (ELISA) were determined.. Trends of decreasing UWS and SWS flow rates and total IgA levels were observed. At days 7 and 11, increases in lactoferrin and albumin levels were found in UWS and SWS. A positive correlation was found between OMNI scores and albumin and lactoferrin levels in SWS (R. Compositional changes in saliva reflecting inflammation were found in the first days after HSCT, and the use of cryotherapy in the second part was associated with decreased OM severity.

Topics: Adult; Aged; Albumins; Cryotherapy; Female; Hematopoietic Stem Cell Transplantation; Humans; Immunoglobulin A; Lactoferrin; Male; Melphalan; Middle Aged; Mucin-5B; Multiple Myeloma; Myeloablative Agonists; Peroxidase; Saliva; Severity of Illness Index; Stomatitis; Transplantation, Autologous

Topics: Ceruloplasmin; Chemotaxis, Leukocyte; Chromosome Inversion; Chromosomes, Human, Pair 3; Female; Humans; Lactoferrin; Middle Aged; Multiple Myeloma; Receptors, Transferrin; Sweet Syndrome

It was recognized that in human milk some amounts of lactoferrin (LF) were naturally bound to secretory IgA (sIgA). Since not only secretory component (SC) but also LF was released from sIgA by disulfide bond cleavage, it is conceivable that LF is naturally bound to IgA as well as SC. An in vitro binding test to LF and IgA was performed and the binding was confirmed by the use of an IgA-Sepharose 4B affinity column.

Topics: Chromatography, Affinity; Colostrum; Disulfides; Female; Humans; Immunoglobulin A, Secretory; Lactoferrin; Lactoglobulins; Milk, Human; Multiple Myeloma; Secretory Component