lactoferrin and Abscess

The efficacy of prophylactic treatment with human lactoferrin 1-11 (hLF1-11), a broad-spectrum antimicrobial peptide, was studied in a rabbit model of femur infection.. Calcium phosphate cement with 50 mg/g hLF1-11 or gentamicin was injected into the femoral canal, after inoculation with Staphylococcus aureus. Three weeks later, slices of the proximal femora were sawn for quantitative bacterial culture and histology.. Treatment with hLF1-11 (P<0.038) or gentamicin (P<0.008) caused a reduction of cfu compared with the untreated control rabbits. The number of sterile cultures was higher in hLF1-11- (3/7) and gentamicin- (5/6) treated animals than in controls (1/7). Radiological and histological analysis showed early bone ingrowth into the cement cracks, and only moderate pathological changes in rabbits with positive cultures.. Local prophylaxis with hLF1-11 effectively reduced development of osteomyelitis in a rabbit model, but gentamicin resulted in a larger number of sterile femora.

Topics: Abscess; Animals; Anti-Bacterial Agents; Anti-Infective Agents; Bone Cements; Calcium Phosphates; Colony Count, Microbial; Drug Carriers; Female; Femur; Gentamicins; Lactoferrin; Methicillin Resistance; Necrosis; Osteogenesis; Osteomyelitis; Peptide Fragments; Rabbits

Topics: Abscess; Adult; Cell Adhesion; Cell Aggregation; Cell Membrane; Chediak-Higashi Syndrome; Chemotaxis, Leukocyte; Granulocytes; Humans; Lactoferrin; Lactoglobulins; Male; Microscopy, Electron; Phagocyte Bactericidal Dysfunction; Recurrence; Skin Diseases