ku-55933 and Parkinson-Disease

In the present study we demonstrated that neurotoxin MPP(+)-induced DNA damage is followed by ataxia telangiectasia muted (ATM) activation either in cerebellar granule cells (CGC) or in B65 cell line. In CGC, the selective ATM inhibitor KU-55933 showed neuroprotective effects against MPP(+)-induced neuronal cell loss and apoptosis, lending support to the key role of ATM in experimental models of Parkinson's disease. Likewise, we showed that knockdown of ATM levels in neuroblastoma B65 cells using an ATM-specific siRNA attenuates the phosphorylation of retinoblastoma protein without affecting other cell-cycle proteins involved in the G(0)/G(1) cell-cycle phase. Moreover, we demonstrated DNA damage, in human brain samples of PD patients. These findings support a model in which MPP(+) leads to ATM activation with a subsequent DNA damage response and activation of pRb. Therefore, this study demonstrates a new link between DNA damage by MPP(+) and cell-cycle re-entry through retinoblastoma protein phosphorylation.

Topics: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; Aged; Aged, 80 and over; Animals; Apoptosis; Ataxia Telangiectasia Mutated Proteins; Brain; Cell Cycle; Cell Cycle Proteins; Cell Line; Cells, Cultured; Cerebellum; DNA Damage; DNA-Binding Proteins; Female; Humans; Male; Middle Aged; Morpholines; Neurotoxins; Parkinson Disease; Protein Serine-Threonine Kinases; Pyrones; Rats; Rats, Sprague-Dawley; Tumor Suppressor Proteins