krn-7000 and Body-Weight

krn-7000 has been researched along with Body-Weight* in 2 studies

Other Studies

2 other study(ies) available for krn-7000 and Body-Weight

ArticleYear
A detrimental role for invariant natural killer T cells in the pathogenesis of experimental dengue virus infection.
    The American journal of pathology, 2011, Volume: 179, Issue:4

    Dengue virus (DENV), a member of the mosquito-borne flaviviruses, is a serious public health problem in many tropical countries. We assessed the in vivo physiologic contribution of invariant natural killer T (iNKT) cells, a population of nonconventional lipid-reactive αβ T lymphocytes, to the host response during experimental DENV infection. We used a mouse-adapted DENV serotype 2 strain that causes a disease that resembles severe dengue in humans. On DENV challenge, splenic and hepatic iNKT cells became activated insofar as CD69 and Fas ligand up-regulation and interferon-γ production. C57BL/6 mice deficient in iNKT cells (Jα18(-/-)) were more resistant to lethal infection than were wild-type animals, and the phenotype was reversed by adoptive transfer of iNKT cells to Jα18(-/-) animals. The absence of iNKT cells in Jα18(-/-) mice was associated with decreased systemic and local inflammatory responses, less liver injury, diminished vascular leak syndrome, and reduced activation of natural killer cells and neutrophils. iNKT cell functions were not necessary for control of primary DENV infection, after either natural endogenous activation or exogenous activation with the canonical iNKT cell agonist α-galactosylceramide. Together, these data reveal a novel and critical role for iNKT cells in the pathogenesis of severe experimental dengue disease.

    Topics: Animals; Body Weight; Cytokines; Dengue; Dengue Virus; Female; Galactosylceramides; Inflammation Mediators; Lymphocyte Activation; Mice; Mice, Inbred C57BL; Natural Killer T-Cells; Neutrophil Activation; Survival Analysis; Viral Load; Virus Replication

2011
α-Galactosylceramide protects mice from lethal Coxsackievirus B3 infection and subsequent myocarditis.
    Clinical and experimental immunology, 2010, Volume: 162, Issue:1

    Myocarditis is an inflammation of the myocardium which often follows virus infections. Coxsackievirus B3 (CVB3), as a marker of the enterovirus group, is one of the most important infectious agents of virus-induced myocarditis. Using a CVB3-induced myocarditis model, we show that injection α-galactosylceramide (α-GalCer), a ligand for invariant natural killer (NK) T (iNK T) cells, can protect the mice from viral myocarditis. After the systemic administration of α-GalCer in CVB3 infected mice, viral transcription and titres in mouse heart, sera and spleen were reduced, and the damage to the heart was ameliorated. This is accompanied by a better disease course with an improved weight loss profile. Compared with untreated mice, α-GalCer-treated mice showed high levels of interferon (IFN)-γ and interleukin (IL)-4, and reduced proinflammatory cytokines and chemokines in their cardiac tissue. Anti-viral immune response was up-regulated by α-GalCer. Three days after CVB3 infection, α-GalCer-administered mice had larger spleens. Besides NK T cells, more macrophages and CD8(+) T cells were found in these spleens. Upon stimulation with phorbol myristate acetate plus ionomycin, splenocytes from α-GalCer-treated mice produced significantly more cytokines [including IFN-γ, tumour necrosis factor-α, IL-4 and IL-10] than those from untreated mice. These data suggest that administration of α-GalCer during acute CVB3 infection is able to protect the mice from lethal myocarditis by local changes in inflammatory cytokine patterns and enhancement of anti-viral immune response at the early stage. α-GalCer is a potential candidate for viral myocarditis treatment. Our work supports the use of anti-viral treatment early to reduce the incidence of virus-mediated heart damage.

    Topics: Animals; Body Weight; Cells, Cultured; Coxsackievirus Infections; Cytokines; Enterovirus B, Human; Enzyme-Linked Immunosorbent Assay; Galactosylceramides; Heart; Host-Pathogen Interactions; Macrophages; Male; Mice; Mice, Inbred BALB C; Myocarditis; Myocardium; Natural Killer T-Cells; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Survival Rate; T-Lymphocytes; Transcription, Genetic; Viral Load

2010