kopsinine and Chemical-and-Drug-Induced-Liver-Injury

Kopsinine 1 mmol/L inhibited microsomal lipid peroxidation (MDA) formation induced by CCl4 in vitro. Metyrapone, a specific inhibitor of cytochrome P-450, partially counteracted the inhibitory effect of kopsinine on MDA formation. Oral administration of kopsinine 200 mg/kg decreased the formation of diene conjugates in liver microsomes in CCl4 (1 ml/kg ig) intoxicated mice. Kopsinine also inhibited 14CCl4 covalent binding to lipids and proteins of liver microsomes. But the degree of inhibition was not as pronounced as that of inhibition of MDA. The metabolic conversion of CCl4 to carbon monoxide and the utilization of NADPH in the course of CCl4 metabolism by liver microsomes were increased by kopsinine in intro. It appears that to maintain the stability of membranes of liver cells is the mode of the protective action of kopsinine.

Topics: Alkaloids; Animals; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Indole Alkaloids; Lipid Peroxidation; Malondialdehyde; Microsomes, Liver; Rats; Rats, Inbred Strains

Kopsinine is an indole alkaloid. Kopsinine 200 mg/kg is significantly protected mice from liver injury induced by CCl4, paracetamol, thioacetamide and prednisolone. The elevated serum transaminase levels and liver lesions were reduced by kopsinine. In addition, kopsinine markedly promoted liver regeneration and the incorporation of [3H]TdR and [3H]leucine into liver protein and DNA, respectively, in partially hepatectomized mice. The results indicate that kopsinine has a hepatoprotective action in mice.

Topics: Alkaloids; Animals; Carbon Tetrachloride; Chemical and Drug Induced Liver Injury; Indole Alkaloids; Liver Function Tests; Liver Regeneration; Male; Mice