kn-93 and Contusions

In the present study, we examined superoxide-mediated excitatory nociceptive transmission on at-level neuropathic pain following spinal thoracic 10 contusion injury (SCI) in male Sprague Dawley rats.. Mechanical sensitivity at body trunk, neuronal firing activity, and expression of superoxide marker/ionotropic glutamate receptors (iGluRs)/CamKII were measured in the T7/8 dorsal horn, respectively.. Topical treatment of superoxide donor t-BOOH (0.4 mg/kg) increased neuronal firing rates and pCamKII expression in the naïve group, whereas superoxide scavenger Tempol (1 mg/kg) and non-specific ROS scavenger PBN (3 mg/kg) decreased firing rates in the SCI group (*. Superoxide and the pCamKII pathway contribute to chronic at-level neuropathic pain without involvement of iGluRs following SCI.

Topics: Animals; Benzylamines; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Contusions; Cyclic N-Oxides; Free Radical Scavengers; Hyperalgesia; Male; Models, Animal; Nerve Tissue Proteins; Neuralgia; Nociception; Pain Threshold; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Receptors, Ionotropic Glutamate; Spin Labels; Spinal Cord Dorsal Horn; Spinal Cord Injuries; Sulfonamides; Superoxides; Synaptic Transmission