kisspeptin-10-protein--human and Neoplasm-Metastasis

kisspeptin-10-protein--human has been researched along with Neoplasm-Metastasis* in 2 studies

Other Studies

2 other study(ies) available for kisspeptin-10-protein--human and Neoplasm-Metastasis

Article	Year
Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor. Nature, 2001, May-31, Volume: 411, Issue:6837 Metastasis is a major cause of death in cancer patients and involves a multistep process including detachment of cancer cells from a primary cancer, invasion of surrounding tissue, spread through circulation, re-invasion and proliferation in distant organs. KiSS-1 is a human metastasis suppressor gene, that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. However, its gene product and functional mechanisms have not been elucidated. Here we show that KiSS-1 (refs 1, 4) encodes a carboxy-terminally amidated peptide with 54 amino-acid residues, which we have isolated from human placenta as the endogenous ligand of an orphan G-protein-coupled receptor (hOT7T175) and have named 'metastin'. Metastin inhibits chemotaxis and invasion of hOT7T175-transfected CHO cells in vitro and attenuates pulmonary metastasis of hOT7T175-transfected B16-BL6 melanomas in vivo. The results suggest possible mechanisms of action for KiSS-1 and a potential new therapeutic approach. Topics: Amino Acid Sequence; Animals; CHO Cells; Cricetinae; Female; Genes, Tumor Suppressor; GTP-Binding Proteins; Humans; Kisspeptins; Ligands; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Neoplasm Metastasis; Peptides; Proteins; Rats; Receptors, Cell Surface; Tissue Distribution; Transfection; Tumor Cells, Cultured; Tumor Suppressor Proteins	2001
The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. The Journal of biological chemistry, 2001, Sep-14, Volume: 276, Issue:37 Natural peptides displaying agonist activity on the orphan G protein-coupled receptor GPR54 were isolated from human placenta. These 54-, 14,- and 13-amino acid peptides, with a common RF-amide C terminus, derive from the product of KiSS-1, a metastasis suppressor gene for melanoma cells, and were therefore designated kisspeptins. They bound with low nanomolar affinities to rat and human GPR54 expressed in Chinese hamster ovary K1 cells and stimulated PIP(2) hydrolysis, Ca(2+) mobilization, arachidonic acid release, ERK1/2 and p38 MAP kinase phosphorylation, and stress fiber formation but inhibited cell proliferation. Human GPR54 was highly expressed in placenta, pituitary, pancreas, and spinal cord, suggesting a role in the regulation of endocrine function. Stimulation of oxytocin secretion after kisspeptin administration to rats confirmed this hypothesis. Topics: Amino Acid Sequence; Animals; CHO Cells; Cricetinae; Genes, Tumor Suppressor; GTP-Binding Proteins; Humans; Kisspeptins; Ligands; Molecular Sequence Data; Neoplasm Metastasis; Proteins; Rats; Receptors, G-Protein-Coupled; Receptors, Kisspeptin-1; Receptors, Neuropeptide; Tumor Suppressor Proteins	2001

Article

Year

Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor.

Nature, 2001, May-31, Volume: 411, Issue:6837

Metastasis is a major cause of death in cancer patients and involves a multistep process including detachment of cancer cells from a primary cancer, invasion of surrounding tissue, spread through circulation, re-invasion and proliferation in distant organs. KiSS-1 is a human metastasis suppressor gene, that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. However, its gene product and functional mechanisms have not been elucidated. Here we show that KiSS-1 (refs 1, 4) encodes a carboxy-terminally amidated peptide with 54 amino-acid residues, which we have isolated from human placenta as the endogenous ligand of an orphan G-protein-coupled receptor (hOT7T175) and have named 'metastin'. Metastin inhibits chemotaxis and invasion of hOT7T175-transfected CHO cells in vitro and attenuates pulmonary metastasis of hOT7T175-transfected B16-BL6 melanomas in vivo. The results suggest possible mechanisms of action for KiSS-1 and a potential new therapeutic approach.

Topics: Amino Acid Sequence; Animals; CHO Cells; Cricetinae; Female; Genes, Tumor Suppressor; GTP-Binding Proteins; Humans; Kisspeptins; Ligands; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Neoplasm Metastasis; Peptides; Proteins; Rats; Receptors, Cell Surface; Tissue Distribution; Transfection; Tumor Cells, Cultured; Tumor Suppressor Proteins

2001

The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54.

The Journal of biological chemistry, 2001, Sep-14, Volume: 276, Issue:37

Natural peptides displaying agonist activity on the orphan G protein-coupled receptor GPR54 were isolated from human placenta. These 54-, 14,- and 13-amino acid peptides, with a common RF-amide C terminus, derive from the product of KiSS-1, a metastasis suppressor gene for melanoma cells, and were therefore designated kisspeptins. They bound with low nanomolar affinities to rat and human GPR54 expressed in Chinese hamster ovary K1 cells and stimulated PIP(2) hydrolysis, Ca(2+) mobilization, arachidonic acid release, ERK1/2 and p38 MAP kinase phosphorylation, and stress fiber formation but inhibited cell proliferation. Human GPR54 was highly expressed in placenta, pituitary, pancreas, and spinal cord, suggesting a role in the regulation of endocrine function. Stimulation of oxytocin secretion after kisspeptin administration to rats confirmed this hypothesis.

Topics: Amino Acid Sequence; Animals; CHO Cells; Cricetinae; Genes, Tumor Suppressor; GTP-Binding Proteins; Humans; Kisspeptins; Ligands; Molecular Sequence Data; Neoplasm Metastasis; Proteins; Rats; Receptors, G-Protein-Coupled; Receptors, Kisspeptin-1; Receptors, Neuropeptide; Tumor Suppressor Proteins

2001