kiss1-protein--human and Carcinoma-in-Situ

The expression profiles of nine bladder cancer cell lines were compared against a pool containing equal total RNA quantities of each of them. Lower expression of KiSS-1 was revealed in cells derived from the most advanced bladder tumors. When comparing 15 primary bladder tumors versus a pool of four bladder cancer cell lines, lower transcript levels of KiSS-1 were observed in the invasive bladder carcinomas as compared to superficial tumors. KiSS-1 expression ratios provided prognostic information. The expression pattern of KiSS-1 transcripts was analyzed using in situ hybridization in nine bladder cancer cells, paired normal urothelium and bladder tumor samples (n = 25), and tissue microarrays of bladder tumors (n = 173). We observed complete loss of KiSS-1 in all invasive tumors under study as compared to their respective normal urothelium. The expression of KiSS-1 was found to be significantly associated with histopathological stage. Patients with lower KiSS-1 expression showed a direct correlation with overall survival in a subset of bladder tumors whose follow-up was available (n = 69). We did not observe any significant differential KiSS-1 expression along cell cycle by sorting analysis. A potential tumor suppressor role in bladder cancer was revealed for KiSS-1. Moreover, it showed predictive value by identifying patients with poor outcome.

Topics: Adult; Aged; Aged, 80 and over; Carcinoma in Situ; Disease Progression; Female; Genes, p53; Genes, Retinoblastoma; Genes, Tumor Suppressor; Humans; In Situ Hybridization; Kisspeptins; Male; Middle Aged; Neoplasm Staging; Oligonucleotide Array Sequence Analysis; Proteins; Smoking; Survival Analysis; Time Factors; Treatment Outcome; Tumor Cells, Cultured; Tumor Suppressor Proteins; Urinary Bladder Neoplasms