kiss1-protein--human and Abortion--Spontaneous

A highly accurate serum marker for predicting viable pregnancy needs to be developed. Recent studies have demonstrated that kisspeptin is a potential biomarker for this purpose.. This systematic review evaluated the available data in the literature on the role of kisspeptin as a miscarriage biomarker. A literature search was conducted in the PubMed/Medline, Embase, Web of Science, and Scopus databases using the following keywords: (kisspeptin) AND (miscarriage OR pregnancy loss OR spontaneous abortion OR reproductive failure).. Seven case-control studies were selected for the systematic review. The included papers described the potential role of kisspeptin as a putative biomarker of pregnancy loss. Furthermore, two studies reported that changes in kisspeptin levels may be associated with unexplained infertility and low rates of embryo implantation in women undergoing assisted reproductive technology.. Kisspeptin might be used as a potential biomarker of pregnancy viability in the near future. However, studies with better evidence are needed to establish the applicability of kisspeptin as a diagnostic and prognostic tool.

Topics: Abortion, Spontaneous; Biomarkers; Female; Fertilization in Vitro; Humans; Infertility; Kisspeptins; Live Birth; Pregnancy; Pregnancy Rate; Reproductive Techniques, Assisted

The objective of this study is to investigate whether kisspeptin levels in early pregnancy have a better diagnostic value on early pregnancy outcome as compared with human chorionic gonadotropin (hCG). This study was a systematic review and meta-analysis aiming to investigate the diagnostic value of kisspeptin levels on early pregnancy outcome. The primary outcome was miscarriage or viable intrauterine pregnancy. Five studies were included for systematic review, and three studies were included for meta-analysis. Meta-analysis showed kisspeptin levels had a good diagnostic value with the area under the curve (AUC) 0.902 (0.866, 0.937) when kisspeptin was measured after 6 weeks of gestation. Sensitivity analysis demonstrated kisspeptin levels had a diagnostic value with AUC = 0.881 (0.855, 0.906). hCG levels had a diagnostic value with AUC = 0.834 (0.785, 0.883), which was inferior to the diagnostic value of kisspeptin (mean difference = 0.09 (0.02, 0.16)). Kisspeptin measurement has a potential for comparable or even higher accuracy than hCG in differentiating between miscarriage and viable intrauterine pregnancy after 6 weeks of gestation.

Topics: Abortion, Spontaneous; Chorionic Gonadotropin; Female; Humans; Kisspeptins; Pregnancy; Pregnancy Outcome; Pregnancy Trimester, First

Kisspeptins are the family of neuropeptide products of the

Topics: Abortion, Spontaneous; Diabetes Mellitus, Type 2; Diabetes, Gestational; Female; Humans; Infant, Newborn; Kisspeptins; Placenta; Pre-Eclampsia; Pregnancy; Pregnancy Complications; Premature Birth

Kisspeptins are a family of neuropeptides that are critical for the puberty initiation and female fertility. Plasma or serum kisspeptin is mainly derived from the placenta during pregnancy and plasma kisspeptin levels significantly increase across pregnancy. Plasma kisspeptin levels could be used as a potential biomarker for the detection of miscarriage, pre-eclampsia, gestational trophoblastic neoplasia (GTN), and fetal development. Kisspeptin may also be involved in the process of parturition by stimulating oxytocin secretion during term pregnancy. This review discussed the potential use of kisspeptin as a marker across pregnancy and highlighted the unresolved problems in this area. Tweetable abstract: Plasma kisspeptin levels could be used as a potential biomarker across pregnancy.

Topics: Abortion, Spontaneous; Biomarkers; Female; Fetal Development; Gestational Trophoblastic Disease; Humans; Kisspeptins; Pre-Eclampsia; Pregnancy

Can serum kisspeptin levels 14 and 21 days after frozen-thawed embryo transfer predict the early pregnancy outcome of patients?. Prospective study, with 133 patients undergoing frozen-thawed embryo transfer. Patients were divided into non-pregnant group and pregnant group (including biochemical pregnancy, singleton pregnancy, miscarriage and twin groups).. Serum kisspeptin levels on day 21 were significantly higher than day 14 in singleton pregnancy, miscarriage and twin groups (all P < 0.0001), but not in the biochemical pregnancy group. Similarly, serum human chorionic gonadotrophin (HCG) levels were higher on day 21 compared with day 14 except for the biochemical pregnancy group. Compared with the twin group (296.9 pg/ml), the other four groups showed significantly higher serum kisspeptin levels on day 14 (non-pregnant 548.9, biochemical pregnancy 440.4, miscarriage 434.9, singleton pregnancy group 420.9 pg/ml, P < 0.01, P = 0.016, P = 0.034, P = 0.036, respectively). The miscarriage (762.2 pg/ml), singleton pregnancy (730.8 pg/ml) and twin groups (826.3 pg/ml) had significantly higher kisspeptin levels than the biochemical pregnancy group (397.3 pg/ml) on day 21 (P < 0.001, P < 0.01, P < 0.001, respectively). Serum kisspeptin levels on day 14 were negatively correlated with embryo implantation rate (P = 0.035, R. Serum kisspeptin levels on day 14 are negatively correlated with embryo implantation rate. Serum kisspeptin levels on day 21 have a poor predictive value of miscarriage.

Topics: Abortion, Spontaneous; Adult; Chorionic Gonadotropin; Cryopreservation; Embryo Implantation; Embryo Transfer; Female; Freezing; Humans; Infertility; Kisspeptins; Predictive Value of Tests; Pregnancy; Pregnancy Outcome; Pregnancy Rate; Time Factors; Young Adult

This study aimed to study the expression and function of kisspeptin during human uterine decidualization in recurrent spontaneous abortion (RSA) and the underlying mechanism.. All patients were recruited from the Clinical Reproductive Center of the Second Affiliated Hospital of Soochow University. Mice models of RSA (CBA/J × DBA/2) and normal pregnancy (CBA/J × BALB/c) were established, respectively. Kisspeptin expression in the serum and decidual tissues of women with RSA were detected. The function of kisspeptin during decidualization in human endometrial stromal cells (HESCs) was assessed by enhancing and silencing kisspeptin expression. CBA/J × DBA/2 pregnant mice were injected with kisspeptin polypeptide, kisspeptin receptor blocker, and expression of decidualization markers was observed. The regulation of ERK1/2 signalling pathway were verified.. Serum kisspeptin levels were significantly lower in patients with RSA than in normal pregnant individuals, as was the expression of kisspeptin, p-ERK, and decidualization indicators in the decidua. Additionally, kisspeptin inhibition downregulated the expression of decidualization markers in HESCs. In mice with RSA, kisspeptin was significantly downregulated, and p-ERK expression at the maternal-foetal interface was significantly decreased. Moreover, exogenous kisspeptin supplementation improved the levels of IGFBP-1 and dPRL, upregulated p-ERK expression, and reduced the abortion rate.. Kisspeptin is involved in promoting uterine decidualization via the ERK1/2 signalling pathway.

Topics: Abortion, Habitual; Abortion, Spontaneous; Animals; Decidua; Female; Humans; Kisspeptins; MAP Kinase Signaling System; Mice; Mice, Inbred CBA; Mice, Inbred DBA; Pregnancy; Stromal Cells

Miscarriage is a major concern in early pregnancy among women having conceived with assisted reproductive treatments. This study aimed to examine potential miscarriage-related biophysical and biochemical markers at 6 weeks' gestation among women with confirmed clinical pregnancy following in vitro fertilization (IVF)/embryo transfer (ET) and evaluate the performance of a model combining maternal factors, biophysical and biochemical markers at 6 weeks' gestation in the prediction of first trimester miscarriage among singleton pregnancies following IVF/ET.. A prospective cohort study was conducted in a teaching hospital between December 2017 and January 2020 including women who conceived through IVF/ET. Maternal mean arterial pressure, ultrasound markers including mean gestational sac diameter, fetal heart activity, crown rump length and mean uterine artery pulsatility index (mUTPI) and biochemical biomarkers including maternal serum soluble fms-like tyrosine kinase-1 (sFlt-1), placental growth factor (PlGF), kisspeptin and glycodelin-A were measured at 6 weeks' gestation. Logistic regression analysis was carried out to determine significant predictors of miscarriage prior to 13 weeks' gestation and performance of screening was estimated by receiver-operating characteristics curve analysis.. Among 169 included pregnancies, 145 (85.8%) pregnancies progressed to beyond 13 weeks' gestation and had live births whereas 24 (14.2%) pregnancies resulted in a miscarriage during the first trimester. In the miscarriage group, compared to the live birth group, maternal age, body mass index, and mean arterial pressure were significantly increased; mean gestational sac diameter, crown rump length, mUTPI, serum sFlt-1, glycodelin-A, and the rate of positive fetal heart activity were significantly decreased, while no significant differences were detected in PlGF and kisspeptin. Significant prediction for miscarriage before 13 weeks' gestation was provided by maternal age, fetal heart activity, mUTPI, and serum glycodelin-A. The combination of maternal age, ultrasound (fetal heart activity and mUTPI), and biochemical (glycodelin-A) markers achieved the highest area under the curve (AUC: 0.918, 95% CI 0.866-0.955), with estimated detection rates of 54.2% and 70.8% for miscarriage before 13 weeks' gestation, at fixed false positive rates of 5% and 10%, respectively.. A combination of maternal age, fetal heart activity, mUTPI, and serum glycodelin-A at 6 weeks' gestation could effectively identify IVF/ET pregnancies at risk of first trimester miscarriage.

Topics: Abortion, Spontaneous; Biomarkers; Female; Gestational Age; Glycodelin; Humans; Infant; Kisspeptins; Placenta Growth Factor; Pre-Eclampsia; Pregnancy; Prospective Studies; Pulsatile Flow; Reproductive Techniques, Assisted; Uterine Artery; Vascular Endothelial Growth Factor Receptor-1

To compare the performance of kisspeptin and beta human chorionic gonadotropin (βhCG), both alone and in combination, as biomarkers for miscarriage throughout the first trimester.. Prospective, nested case-control study.. Tertiary Centre, Queen Charlotte Hospital, London, United Kingdom.. Adult women who had miscarriages (n = 95, 173 samples) and women with healthy pregnancies (n = 265, 557 samples).. The participants underwent serial ultrasound scans and blood sampling for measurement of plasma kisspeptin and βhCG levels during the first trimester.. The ability of plasma kisspeptin and βhCG levels to distinguish pregnancies complicated by miscarriage from healthy pregnancies unaffected by miscarriage.. Gestation-adjusted levels of circulating kisspeptin and βhCG were lower in samples from women with miscarriages than in women with healthy pregnancies by 79% and 70%, respectively. The area under the receiver-operating characteristic curve for identifying miscarriage during the first trimester was 0.874 (95% confidence interval [CI] 0.844-0.904) for kisspeptin, 0.859 (95% CI 0.820-0.899) for βhCG, and 0.916 (95% CI 0.886-0.946) for the sum of the two markers. The performance of kisspeptin in identifying miscarriage improved with increasing length of gestation, whereas that of βhCG worsened. A decision matrix incorporating kisspeptin, βhCG, and gestational age had 83% to 87% accuracy for the prediction of miscarriage.. Plasma kisspeptin is a promising biomarker for miscarriage and provides additional value to βhCG alone, especially during later gestational weeks of the first trimester.

Topics: Abortion, Spontaneous; Biomarkers; Case-Control Studies; Chorionic Gonadotropin, beta Subunit, Human; Down-Regulation; Female; Gestational Age; Humans; Kisspeptins; Predictive Value of Tests; Pregnancy; Pregnancy Trimester, First; Prospective Studies; Ultrasonography, Prenatal

Topics: Abortion, Spontaneous; Biomarkers; Female; Humans; Kisspeptins; Pregnancy; Pregnancy Trimester, First

What is the diagnostic value of maternal kisspeptin in patients with asymptomatic first-trimester pregnancies, and what is the prognostic significance of kisspeptin versus beta-HCG in early pregnancies.. Case-control study in academic medical centres. Patients with no confounding co-morbidities who conceived by IVF and intracytoplasmic sperm injection were analysed. Maternal serum samples were assessed at the time of pregnancy testing. Women who achieved a positive pregnancy test were asked to take serum samples 4 days later. According to the follow-up results, patients who experienced biochemical pregnancy loss (n = 24) and early miscarriage (n = 21), and women who achieved a viable pregnancy (n = 28), were included in this study. Serum samples were collected to detect kisspeptin and beta-HCG, respectively.. Single serum determinations of kisspeptin and beta-HCG were correlated with the different pregnancy outcomes. Women who experienced biochemical pregnancy loss showed lower kisspeptin levels than those in groups B and C. No significant difference, however, was observed at the time of pregnancy testing in women who had experienced early miscarriage and those who had achieved viable pregnancy. Sequential measurements of serum kisspeptin are not as effective as beta-HCG in determining pregnancy outcome. Increased kisspeptin level was associated with reduced miscarriage risk.. Single serum measurement of kisspeptin is significantly altered in pregnant and non-pregnant women. However, it failed to discriminate between miscarriage and ongoing pregnancies in first-trimester pregnancy. Neither single nor sequential kisspeptin have higher diagnostic performance for miscarriage than beta-HCG in early stage.

Topics: Abortion, Spontaneous; Biomarkers; Case-Control Studies; Chorionic Gonadotropin, beta Subunit, Human; Female; Fertilization in Vitro; Gestational Age; Humans; Kisspeptins; Pregnancy; Pregnancy Outcome; Pregnancy Trimester, First; Prognosis; Sperm Injections, Intracytoplasmic

Topics: Abortion, Spontaneous; Biomarkers; Female; Humans; Kisspeptins; Pregnancy

To validate the ability of serum kisspeptin-54 to discriminate between first-trimester viable pregnancies and miscarriages.. Case-control study.. Academic medical centers.. Women with confirmed viable intrauterine pregnancy (IUP) at estimated gestational age 6-10 weeks (n = 20), women with confirmed miscarriage (spontaneous abortion [SAB]) at estimated gestational age 6-10 weeks (n = 20), and nonpregnant women (n = 19).. Collection of serum samples from women with confirmed IUP, SAB, and nonpregnant women for the measurement of serum kisspeptin and serum hCG levels.. Serum kisspeptin and hCG.. The limit of detection was 0.024 ng/mL; intra- and interassay coefficients of variation were 5.1% and 8.6%, respectively. Kisspeptin levels differed between the pregnant and nonpregnant state and by viability. Kisspeptin levels were positively associated with gestational age. There was also a significant positive association with hCG in SAB, but not in IUP.. Plasma levels of kisspeptin have been suggested as a biomarker for miscarriage. This study demonstrates kisspeptin assay stability in serum and its potential clinical utility as a biomarker for early pregnancy viability.

Topics: Abortion, Spontaneous; Adult; Biomarkers; Case-Control Studies; Chorionic Gonadotropin; Cross-Sectional Studies; Diagnosis, Differential; Female; Gestational Age; Humans; Kisspeptins; Limit of Detection; Predictive Value of Tests; Pregnancy; Pregnancy Tests; Pregnancy Trimester, First; Reproducibility of Results; Young Adult

Kisspeptin is a recently identified hormone encoded by the KISS1 gene, playing a critical role in human reproduction. Plasma kisspeptin levels rise dramatically during normal pregnancy due to placental synthesis, which implicates it as a potential tool for assessing risks of pregnancy complications. No previous prospective study has investigated the association between plasma kisspeptin and risk of miscarriage.. The objective of the study was to determine whether a single plasma kisspeptin or serum human chorionic gonadotropin (hCG) measurement in asymptomatic women attending their booking antenatal visit is associated with miscarriage.. This was a prospective cohort study.. The study was conducted at a tertiary obstetric center.. A total of 993 asymptomatic pregnant women with a gestation of 6 weeks or longer attending routine antenatal booking visit were recruited between January 2010 and December 2012.. Plasma kisspeptin and serum hCG were measured during the antenatal booking visit. Pregnancy outcome was recorded prospectively.. Plasma kisspeptin correlated with gestation (r(2) = 0.57; P < .0001). Gestational age-corrected (multiples of median) plasma kisspeptin was 60.4% lower (P < .001), and multiples of median-hCG was 36.1% lower (P < .001) in women later diagnosed with miscarriage compared with women without miscarriage. Increased plasma kisspeptin was associated with reduced miscarriage risk, even after adjusting for age, body mass index, gestational age, smoking, and blood pressure [odds ratio 0.13 (95% confidence interval 0.08-0.22), P = .0001]. Kisspeptin had a higher diagnostic performance for miscarriage than hCG (receiver-operator characteristic-area under the curve 0.899 ± 0.025 plasma kisspeptin; 0.775 ± 0.040, serum hCG, P < .01 vs plasma kisspeptin).. Our data suggest for the first time that a single plasma kisspeptin measurement taken during the antenatal booking visit provides a potential novel marker for identifying asymptomatic pregnant women at a gestation of 6 weeks or greater at increased risk of miscarriage.

Topics: Abortion, Spontaneous; Adult; Chorionic Gonadotropin; Cohort Studies; Female; Gestational Age; Humans; Kisspeptins; Pregnancy; Pregnancy Trimester, First; Prenatal Care; Prospective Studies; Risk