kiss1-protein--human and Abortion--Habitual

To study choriodecidual immunoreactivity of kisspeptin (KISS1) and its receptor (KISS1R) in recurrent pregnancy loss (RPL) due to aneuploidy (AnE) and unexplained (UE) RPL in comparison to control elective abortions (EAbs).. This is a case-control study.. Tertiary care facility and affiliated research institute.. Patients with either UE RPL (n = 10) or RPL due to AnE (n = 10) vs. a control group of patients who underwent EAb (n = 10).. Immunohistochemistry of archived choriodecidual tissue samples.. Histoscores of KISS1 and KISS1R immunoreactivity in the syncytiotrophoblast (SyT), cytotrophoblast (CyT), decidual glands (DeGs), and decidual stroma (DeS) across the 3 study groups.. There was no difference in both maternal and gestational ages among the 3 groups. Kisspeptin immunoreactivity was similar in the SyT, CyT, DeGs, and DeS of all groups. Similarly, KISS1R expression was not different in the DeGs or DeS among all study groups. In addition, there was no difference in KISS1R immunoreactivity in the SyTs and CyTs between patients with RPL due to AnE and those with UE RPL. However, KISS1R was significantly lower in the SyT and CyT of patients with RPL due to AnE and UE RPL than in those who underwent EAb.. The expression of KISS1R is lower in the chorionic tissues of euploid (unexplained) and aneuploid RPLs than in the control group. The current results broaden our understanding of the role played by KISS1 and KISS1R in early placentation. Further investigation is necessary to determine whether KISS1 activity is the cause or a sequel of defective placentation.

Topics: Abortion, Habitual; Aneuploidy; Case-Control Studies; Female; Humans; Kisspeptins; Pregnancy; Receptors, Kisspeptin-1

This study aimed to study the expression and function of kisspeptin during human uterine decidualization in recurrent spontaneous abortion (RSA) and the underlying mechanism.. All patients were recruited from the Clinical Reproductive Center of the Second Affiliated Hospital of Soochow University. Mice models of RSA (CBA/J × DBA/2) and normal pregnancy (CBA/J × BALB/c) were established, respectively. Kisspeptin expression in the serum and decidual tissues of women with RSA were detected. The function of kisspeptin during decidualization in human endometrial stromal cells (HESCs) was assessed by enhancing and silencing kisspeptin expression. CBA/J × DBA/2 pregnant mice were injected with kisspeptin polypeptide, kisspeptin receptor blocker, and expression of decidualization markers was observed. The regulation of ERK1/2 signalling pathway were verified.. Serum kisspeptin levels were significantly lower in patients with RSA than in normal pregnant individuals, as was the expression of kisspeptin, p-ERK, and decidualization indicators in the decidua. Additionally, kisspeptin inhibition downregulated the expression of decidualization markers in HESCs. In mice with RSA, kisspeptin was significantly downregulated, and p-ERK expression at the maternal-foetal interface was significantly decreased. Moreover, exogenous kisspeptin supplementation improved the levels of IGFBP-1 and dPRL, upregulated p-ERK expression, and reduced the abortion rate.. Kisspeptin is involved in promoting uterine decidualization via the ERK1/2 signalling pathway.

Topics: Abortion, Habitual; Abortion, Spontaneous; Animals; Decidua; Female; Humans; Kisspeptins; MAP Kinase Signaling System; Mice; Mice, Inbred CBA; Mice, Inbred DBA; Pregnancy; Stromal Cells

It is widely accepted that kisspeptin plays an integral role in the regulation of reproduction. Genetic variations in the KISS1 gene have been frequently reported to be linked to reproductive diseases, but there is still a lack of data on the association between KISS1 variations and female reproductive disorders. The present study aimed to examine the association of three missense SNPs in the KISS1 gene including rs12998, rs35431622, and rs4889 in association with idiopathic recurrent pregnancy loss (iRPL). A total of 720 individuals were recruited in this study. The DNA from the blood sample was extracted and genotyped using the PCR method. Haplotype and linkage disequilibrium (LD) have also been analyzed. The results of this study suggested that rs12998 G > A and rs4889 C > G had a significant association with iRPL (p < 0.05); while rs35431622 A > G didn't indicate any association with iRPL. A significant association was also found for three haplotypes including C-A-A, G-G-G, and G-G-A in this population. The analysis also showed a significant LD between rs12998 and rs35431622 (P < 0.0005). The rs12998 G > A and rs4889 C > G variants of KISS1 are linked to unexplained recurrent pregnancy loss and may be risk factors for this disease.

Topics: Abortion, Habitual; Female; Genes, Tumor Suppressor; Genotype; Humans; Kisspeptins; Polymorphism, Single Nucleotide; Pregnancy

Kisspeptin and its receptor GPR54 play a major role in trophoblast invasion, and progesterone-induced blocking factor (PIBF) is needed for maintaining pregnancy. The expression of kisspeptin/GPR54 and PIBF/progesterone receptor (PR) in trophoblasts and deciduas and the relationship between kisspeptin and PIBF were investigated in the same women with recurrent spontaneous abortion (RSA). Trophoblastic and decidual tissues were collected from 32 RSA women who miscarried a genetically normal fetus, and 35 women who had voluntary abortion. Kisspeptin, GPR54, PIBF and PR were investigated using immunohistochemistry. Kisspeptin, GPR54 and PIBF expressions in syncytiotrophoblasts and cytotrophoblasts were decreased in RSA women as compared to controls (P<0.05). Kisspeptin, PIBF and PR expressions in deciduas were significantly decreased in RSA women as compared to controls (P<0.01). GPR54 expression in deciduas nearly showed no difference between the RSA group and the control group (P=0.958). Kisspeptin and PIBF expressions in syncytiotrophoblasts, cytotrophoblasts and deciduas were correlated with each other in the RSA group (Kappa=0.602, P=0.001; Kappa=0.590, P=0.001; Kappa=0.392, P=0.011). These data support the hypothesis that decreased kisspeptin and PIBF expressions in trophoblasts and deciduas are associated with RSA.

Topics: Abortion, Habitual; Adult; Decidua; Female; Humans; Immunohistochemistry; Kisspeptins; Pregnancy; Pregnancy Proteins; Pregnancy Trimester, First; Receptors, G-Protein-Coupled; Receptors, Kisspeptin-1; Receptors, Progesterone; Suppressor Factors, Immunologic; Trophoblasts

Kisspeptin and its receptor GPR54 play a major role in trophoblast invasion. The expression of kisspeptin and GPR54 in trophoblast and decidua and their relationship with decidual and peripheral blood natural killer (NK) cells are investigated in women with RPL.. Trophoblast and decidual tissues were collected from 38 RPL women who miscarried a genetically normal fetus and 14 women who had elective abortion. Kisspeptin, GPR54, and decidual NK cells were investigated with immunohistochemistry, and peripheral blood NK cells were analyzed by flow cytometry.. Kisspeptin expression in syncytiotrophoblast was significantly decreased in RPL women with normal (<15%) peripheral blood NK cells (npNK) (P=0.021) and high (≥15%) peripheral blood NK cells (hpNK) (P=0.024) as compared to controls. Kisspeptin expression in cytotrophoblast was significantly decreased hpNK group (P=0.009) as compared to controls. GPR54 expressions were not different among study groups and controls. The number of CD56(+) decidual NK cells are significantly higher in hpNK group as compared to npNK group (P=0.041) and showed a correlation with kisspeptin expression in syncytiotrophoblasts (r=0.738, P<0.001).. Decreased kisspeptin expression in trophoblasts is associated with RPL and kisspeptin may engage the regulation of decidual NK cell infiltration.

Topics: Abortion, Habitual; Abortion, Induced; CD56 Antigen; Decidua; Female; Humans; Killer Cells, Natural; Kisspeptins; Pregnancy; Pregnancy Complications; Pregnancy Trimester, First; Receptors, G-Protein-Coupled; Receptors, IgG; Receptors, Kisspeptin-1; Trophoblasts