keratan-sulfate and Haemophilus-Infections

Intracisternal or intraarticular inoculation of rabbit recombinant interleukin (IL)-1 beta and rabbit tumor necrosis factor-alpha combined with IL-1 receptor antagonist (IL-1RA) and soluble tumor necrosis factor receptor (sTNFR), respectively, produced significantly less inflammation in rabbits than after inoculation of these cytokines alone. In contrast, when Haemophilus influenzae type b (Hib) or Hib lipooligosaccharide (LOS) was given intraarticularly with IL-1RA, sTNFR, or the combination, there was no significant or consistent modulation of synovial inflammation and cartilage proteoglycan degradation. In the experimental meningitis model, IL-1RA and sTNFR did not significantly reduce the meningeal inflammatory response associated with intracisternal inoculation of Hib LOS. These data indicate that specific cytokine inhibitors (sTNFR and IL-1RA) may not be effective in modulating inflammation induced by a broad inflammatory stimulus such as gram-negative bacteria or their products and suggest caution in using them to treat these infectious conditions in humans.

Topics: Animals; Arthritis, Infectious; Brain Edema; Cerebrospinal Fluid Proteins; Haemophilus Infections; Haemophilus influenzae; Humans; Interleukin 1 Receptor Antagonist Protein; Interleukin-1; Keratan Sulfate; Lactates; Lactic Acid; Leukocyte Count; Lipopolysaccharides; Male; Meningitis, Haemophilus; Rabbits; Receptors, Tumor Necrosis Factor; Sialoglycoproteins; Solubility; Synovial Fluid; Tumor Necrosis Factor-alpha