kb-r7943 and Inflammation

kb-r7943 has been researched along with Inflammation* in 2 studies

Other Studies

2 other study(ies) available for kb-r7943 and Inflammation

ArticleYear
Trafficking of Na+/Ca2+ exchanger to the site of persistent inflammation in nociceptive afferents.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015, Jun-03, Volume: 35, Issue:22

    Persistent inflammation results in an increase in the amplitude and duration of depolarization-evoked Ca(2+) transients in putative nociceptive afferents. Previous data indicated that these changes were the result of neither increased neuronal excitability nor an increase in the amplitude of depolarization. Subsequent data also ruled out an increase in voltage-gated Ca(2+) currents and recruitment of Ca(2+)-induced Ca(2+) release. Parametric studies indicated that the inflammation-induced increase in the duration of the evoked Ca(2+) transient required a relatively large and long-lasting increase in the concentration of intracellular Ca(2+) implicating the Na(+)/Ca(2+) exchanger (NCX), a major Ca(2+) extrusion mechanism activated with high intracellular Ca(2+) loads. The contribution of NCX to the inflammation-induced increase in the evoked Ca(2+) transient in rat sensory neurons was tested using fura-2 AM imaging and electrophysiological recordings. Changes in NCX expression and protein were assessed with real-time PCR and Western blot analysis, respectively. An inflammation-induced decrease in NCX activity was observed in a subpopulation of putative nociceptive neurons innervating the site of inflammation. The time course of the decrease in NCX activity paralleled that of the inflammation-induced changes in nociceptive behavior. The change in NCX3 in the cell body was associated with a decrease in NCX3 protein in the ganglia, an increase in the peripheral nerve (sciatic) yet no change in the central root. This single response to inflammation is associated with changes in at least three different segments of the primary afferent, all of which are likely to contribute to the dynamic response to persistent inflammation.

    Topics: Animals; Antigens, CD; Antigens, Differentiation, Myelomonocytic; Calcium; Disease Models, Animal; Freund's Adjuvant; Ganglia, Spinal; Gene Expression Regulation; Inflammation; Male; Membrane Potentials; Pain Measurement; Patch-Clamp Techniques; Protein Transport; Rats; Rats, Sprague-Dawley; Sciatica; Sensory Receptor Cells; Skin; Sodium-Calcium Exchanger; Thiourea; Time Factors

2015
Potentiated macrophage activation by acid sensing under low adiponectin levels.
    Molecular immunology, 2014, Volume: 57, Issue:2

    Adiponectin can protect against inflammation; one of the mechanisms involves direct, inhibition of macrophages (MΦ). We postulated that adiponectin anti-sense transgenic (AsTg) mice raised in our laboratory are prone to inflammation because of systemic low adiponectin levels. The writhing response to acetic acid was utilized as an in vivo inflammatory model, and using Ca(2)(+), response to the acid was exploited in vitro to evaluate the function of resident peritoneal MΦ. The in vivo response to the acid was increased and the Ca(2)(+) response of MΦ was enhanced in AsTg mice, compared with those in wild type (WT) mice. In parallel with these enhanced responses, MΦ from AsTg mice augmented TNF-α and IL-6 mRNA expression. We further analyzed the enhancement in activity of MΦ from AsTg mice by acid sensing using specific inhibitors, amiloride for acid-sensing ion channels (ASICs) and KB-R7943 for Na(+)/Ca(2)(+) exchangers (NCXs). Our results indicated that in AsTg mice, the Ca(2)(+) response to the acid was facilitated in MΦ by a low threshold of ASIC1 and NCX1 molecules and the activity of these channel was possibly regulated by adiponectin.

    Topics: Acetic Acid; Acid Sensing Ion Channel Blockers; Acid Sensing Ion Channels; Adiponectin; Amiloride; Animals; Anti-Arrhythmia Agents; Calcium; Cell Line; Female; Inflammation; Interleukin-6; Macrophage Activation; Macrophages; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; Peritoneum; RNA, Messenger; Sodium-Calcium Exchanger; Thiourea; Tumor Necrosis Factor-alpha

2014