kaolinite and Lymphoma

To determine whether thromboelastography is more accurate than conventional methods of evaluating hemostasis for the prediction of clinical bleeding in thrombocytopenic dogs following total body irradiation (TBI) and bone marrow transplantation (BMT).. 10 client-owned thrombocytopenic dogs with multicentric lymphoma.. Results of a kaolin-activated thromboelastography assay, platelet count, and buccal mucosal bleeding time were evaluated for correlation to clinical bleeding.. Maximum amplitude, derived via thromboelastography, was the only hemostatic variable with significant correlation to clinical bleeding. Buccal mucosal bleeding time had a high sensitivity but poor specificity for identifying dogs with clinical bleeding.. Compared with buccal mucosal bleeding time and platelet count, thromboelastography was more reliable at identifying thrombocytopenic dogs with a low risk of bleeding and could be considered to help guide the use of transfusion products in dogs undergoing TBI and BMT.

Topics: Animals; Bone Marrow Transplantation; Cohort Studies; Dog Diseases; Dogs; Hemorrhage; Hemostasis; Kaolin; Lymphoma; Male; Platelet Count; Prospective Studies; Thrombelastography; Thrombocytopenia; Whole-Body Irradiation

A cultured tumor cell line from the Greene lymphoma was shown in vitro to secrete immunoglobins of the IgG2 subclass (TcIg), which possessed a direct inhibiting effect on blood coagulation. TcIg prolonged the thrombin time (TT) of normal hamster plasma in vitro. Moreover, the effect of heparin which prolonged the plasma thrombin time was reinforced when TcIg was added to the test. This property was not neutralized by protamine sulfate. In the plasma kaolin clotting time test (KCT), the addition of TcIg lead to an anticoagulant effect, but weaker than in TT. The mixing pattern of normal plasma with tumor-bearing hamster plasma did not reveal the presence of circulating inhibitor in tumor plasma. It was suggested that TcIg had heparin-like activity.

Topics: Animals; Antibodies, Neoplasm; Blood Coagulation; Blood Coagulation Factors; Cell Line; Cricetinae; Heparin; Hydrogen-Ion Concentration; Immunoglobulin G; Kaolin; Lymphoma; Protamines; Thrombin Time