kallidin and Pancreatitis

Topics: Animals; Aprotinin; Blood Vessels; Bradykinin; Brain; Dumping Syndrome; Humans; Kallidin; Kallikreins; Kinins; Liver Cirrhosis; Liver Diseases; Malignant Carcinoid Syndrome; Pancreatitis

Topics: Acute Disease; Animals; Aprotinin; Bradykinin; Dogs; Humans; Kallidin; Kallikreins; Kinetics; Kininogens; Kinins; Pancreatitis; Shock; Trypsin; Trypsin Inhibitors

Topics: Anaphylaxis; Angioedema; Arthritis; Bradykinin; Burns; Dumping Syndrome; Half-Life; Humans; Inflammation; Kallidin; Kallikreins; Kinins; Malignant Carcinoid Syndrome; Pancreatitis; Shock; Shock, Septic; Vascular Diseases

Topics: Anaphylaxis; Animals; Aprotinin; Bradykinin; Cattle; Cerebral Hemorrhage; Dogs; Enteritis; Fibrinolysis; Guinea Pigs; Humans; Kallidin; Kallikreins; Kinins; Mice; Pancreatitis; Rats; Shock, Hemorrhagic; Subarachnoid Hemorrhage

In this study we compare the role of kinin-B1 and B2 receptors during ischaemia/reperfusion of rat pancreas. Our investigations were prompted by the observation that infusion of a kinin-B2 receptor antagonist produced significant improvement in acute experimental pancreatitis. In an acute model with two hours of ischaemia/two hours of reperfusion, application of the kinin-B1 receptor antagonist (CP-0298) alone, or in combination with kinin-B2 receptor antagonist (CP-0597), significantly reduced the number of adherent leukocytes in post-capillary venules. In a chronic model with five days of reperfusion, the continuous application of kinin-B1 receptor antagonist or a combination of kinin-B1 and B2 receptor antagonists markedly reduced the survival rate. In kinin-receptor binding studies kinin-B1 receptor showed a 22-fold increase in expression during the time of ischaemia/reperfusion. Carboxypeptidase M activity was up-regulated 10-fold following two hours of ischaemia and two hours of reperfusion, provided the appropriate specific ligand, des-Arg10-kallidin and/or des-Arg9-bradykinin, was used. The occurrence of kinin-B1 receptor binding sites on acinar cell membranes was demonstrated by micro-autoradiography. With a specific antibody, the localisation of kinin-B1 receptor protein was confirmed at the same sites. In conclusion, we have demonstrated the up-regulation of the pancreatic acinar cell kinin-B1 receptors during ischaemia/reperfusion. The novel functional finding was that antagonism of the kinin-B1 receptors decreased the survival rate in an experimental model of pancreatitis.

Topics: Animals; Bradykinin; Bradykinin B1 Receptor Antagonists; Bradykinin B2 Receptor Antagonists; Cell Adhesion; GPI-Linked Proteins; Kallidin; Leukocytes; Male; Metalloendopeptidases; Pancreatitis; Protein Binding; Rats; Rats, Sprague-Dawley; Receptor, Bradykinin B1; Receptor, Bradykinin B2; Reperfusion Injury; Survival Rate; Up-Regulation; Venules

Topics: Angioedema; Bradykinin; Carboxypeptidases; Fibrinolysis; Humans; Hypotension; Inflammation; Kallidin; Kallikreins; Kinins; Malignant Carcinoid Syndrome; Meningitis; Pancreas; Pancreatitis; Peptide Hydrolases; Salivary Glands; Urticaria

Topics: Animals; Arthritis; Blood Pressure; Bradykinin; Cardiovascular Diseases; Dogs; Edema; Guinea Pigs; Humans; Hypersensitivity; Injections, Intravenous; Kallidin; Kallikreins; Pancreatitis; Shock

Topics: Animals; Bradykinin; Dogs; Female; Histamine; Kallidin; Male; Pancreas; Pancreatitis; Regional Blood Flow; Serotonin