juglanin and Pancreatic-Neoplasms

The inhibition of the Hedgehog (Hh) signaling pathway has emerged as an anti-cancer strategy. Three flavonoid glycosides including 2 new compounds (1-2) were isolated from Excoecaria agallocha as Hedgehog/GLI1-mediated transcriptional inhibitors and exhibited cytotoxicity against human pancreatic (PANC1) and prostate (DU145) cancer cells. Our data revealed that compound 1 clearly inhibited the expression of GLI-related proteins (PTCH and BCL-2) and blocked the translocation of GLI1 transcription factors into the nucleus in PANC1. Deleting the Smoothened (Smo) function in PANC1 treated with 1 led to downregulation of the mRNA expression of Ptch. This study describes the first Hh signaling inhibitor which blocks GLI1 movement into the nucleus without interfering with Smo.

Topics: Antineoplastic Agents, Phytogenic; Cell Line, Tumor; Cell Survival; Euphorbiaceae; Flavonoids; Gene Expression Regulation, Neoplastic; Glycosides; Hedgehog Proteins; Humans; Male; Neoplasms; Pancreatic Neoplasms; Patched Receptors; Patched-1 Receptor; Prostatic Neoplasms; Receptors, Cell Surface; Signal Transduction; Transcription Factors; Transcriptional Activation; Zinc Finger Protein GLI1