jasplakinolide has been researched along with Malaria--Falciparum* in 2 studies
2 other study(ies) available for jasplakinolide and Malaria--Falciparum
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A new model for hemoglobin ingestion and transport by the human malaria parasite Plasmodium falciparum.
The current model for hemoglobin ingestion and transport by intraerythrocytic Plasmodium falciparum malaria parasites shares similarities with endocytosis. However, the model is largely hypothetical, and the mechanisms responsible for the ingestion and transport of host cell hemoglobin to the lysosome-like food vacuole (FV) of the parasite are poorly understood. Because actin dynamics play key roles in vesicle formation and transport in endocytosis, we used the actin-perturbing agents jasplakinolide and cytochalasin D to investigate the role of parasite actin in hemoglobin ingestion and transport to the FV. In addition, we tested the current hemoglobin trafficking model through extensive analysis of serial thin sections of parasitized erythrocytes (PE) by electron microscopy. We find that actin dynamics play multiple, important roles in the hemoglobin transport pathway, and that hemoglobin delivery to the FV via the cytostomes might be required for parasite survival. Evidence is provided for a new model, in which hemoglobin transport to the FV occurs by a vesicle-independent process. Topics: Actin Cytoskeleton; Actins; Animals; Antifungal Agents; Cytochalasin D; Depsipeptides; Endocytosis; Erythrocytes; Hemoglobins; Humans; Malaria, Falciparum; Microscopy, Electron, Transmission; Microtomy; Models, Biological; Nucleic Acid Synthesis Inhibitors; Plasmodium falciparum; Protein Transport; Transport Vesicles; Vacuoles | 2008 |
Effect of jasplakinolide on the growth, invasion, and actin cytoskeleton of Plasmodium falciparum.
The effect of jasplakinolide (JAS), an actin-polymerizing and filament-stabilizing drug, on the growth, invasion, and actin cytoskeleton of Plasmodium falciparum was examined. Jasplakinolide markedly decreased the parasitemia in a synchronized culture of P. falciparum strain FCR-3 in a time- and concentration-dependent manner. The decrease became evident at day 2 at concentrations of 0.3 micro M and above, and parasites finally disappeared at day 4. Giemsa-stained smears of P. falciparum-infected erythrocytes demonstrated that there was no effect on the development of schizonts from ring forms. Merozoites were released from the infected erythrocytes in a normal manner with and without JAS. However, there were no ring form-infected erythrocytes when JAS was administered, even after the release of merozoites. This indicates that the merozoites exposed to JAS failed to invade erythrocytes. The inhibitory effect of JAS on the parasitemia was reversed by the removal of the drug after exposure to 1 micro M of JAS for 1 day. Electron microscopy revealed that the merozoites treated with JAS showed a protrusion of the apical end which contained the microfilament structure. Immunoblot analysis indicated that the JAS treatment increased F-actin filaments of merozoites but had no effect on those of the trophozoites and schizonts. Therefore, this study demonstrated that JAS has an antimalarial activity. Topics: Actin Cytoskeleton; Actins; Animals; Antiprotozoal Agents; Cells, Cultured; Culture Media; Depsipeptides; Electrophoresis, Polyacrylamide Gel; Humans; Malaria, Falciparum; Peptides, Cyclic; Plasmodium falciparum | 2002 |