ix-207-887 and Disease-Models--Animal

The time-course and pharmacological modulation of interleukin-1 (IL-1) production were investigated during zymosan induced peritonitis in mice. IL-1 alpha liberation was assessed by specific immunoassay (ELISA) and the IL-1 like bioactivity (sensitive to both alpha- and beta-forms of IL-1) was measured by a sensitive bioassay (D10G4.1 costimulation). I.p. injection of zymosan induced significant IL-1 release into the peritoneal exudate. The level peaked at 4 h and by 24 h dropped below the detection limit in both assays. The effects of the prototypical antiinflammatory drugs indomethacin (IND) and dexamethasone (DEX) and that of IX 207-887, a compound which has been reported to interfere primarily with IL-1 production, were also tested. DEX and IX 207-887 dose-dependently decreased the immunoassayable IL-1 alpha level and the IL-1 like bioactivity as well. However, IND had no suppressant effect. Thus, the data obtained by immunoassay and bioassay correlated well proving the suitability of zymosan peritonitis model for the examination of IL-1 production in experimental inflammation.

Topics: Analysis of Variance; Animals; Anti-Inflammatory Agents; Anti-Inflammatory Agents, Non-Steroidal; Biological Assay; Dexamethasone; Disease Models, Animal; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Indomethacin; Interleukin-1; Male; Mice; Peritonitis; Thiophenes; Zymosan