isopropyl-thiogalactoside and Toxoplasmosis

This study aims to determine the rates of clinically-significant anxiety and depressive symptoms during the immediate postpartum in a sample of women referred to a university maternity department, as well as the associated risk factors and the relations with the level of maternal bonding.. During the third national lockdown for the COVID-19 pandemic (February-April 2021), on days 2-3 after delivery 127 mothers were administrated the Edinburgh postnatal depression scale (EPDS), the state-trait anxiety inventory (STAI-YA), the mother-to-infant bonding scale (MIBS) and questions issued from the coronavirus health impact survey questionnaire (CRISIS).. The observed rates of anxiety and depressive symptoms were in the same range as those reported in observational studies conducted in high-resource countries during the COVID-19 pandemic. Risk of being infected by the SARS-CoV2 was both an independent risk factor for anxiety and depressive symptoms. The relations between the measure of maternal bonding and the severity of maternal emotional symptoms call for a better consideration of the long-term consequences of the pandemic on children's socio-emotional development.. Cette étude a pour objectif de déterminer la fréquence des symptômes dépressifs et anxieux maternels lors de la période du post-partum immédiat dans un groupe de femmes venant d’accoucher sur un service universitaire, ainsi que les facteurs de risques associés et les conséquences sur le lien mère-enfant.. Au tout début de la pandémie de COVID-19 (entre les mois de février et avril 2021), 127 femmes venant d’accoucher ont complété dans les 2 à 3 jours qui suivent l’accouchement l’Edinburgh postnatal depression scale (EPDS), le State-trait anxiety inventory (STAI-YA), le Mother-to-infant bonding scale (MIBS) et le Coronavirus health impact survey questionnaire (CRISIS).. La fréquence des symptômes dépressif cliniquement significatifs était de 17 % et de 15 % pour les symptômes anxieux. Le risque d’infection par le SARS-CoV2 était associé à la sévérité de ces symptômes dans les analyses multivariées. Des corrélations statistiquement significatives ont été mis en évidence entre le score MIBS et le score EPDS (rs = 0.26) et avec le score STAI-YA (rs = 0.26).. La fréquence des symptômes anxieux et dépressifs du péripartum étaient comparable avec d’autres études conduites dans les pays à haut niveau de ressource au cours de la pandémie de COVID-19. Le risque d’infection par le SARS-CoV2 est associé à des niveaux plus élevés de symptômes anxieux et dépressifs, à côté des autres facteurs connus de symptômes émotionnels du post-partum. Les liens retrouvés entre ces symptômes et le niveau de lien mère-enfant invitent à être attentif aux conséquences à long-terme de la pandémie sur le développement socio-émotionnel du nourrisson.. Des études complémentaires sont nécessaires pour confirmer ces résultats et déterminer les conséquences potentiellement délétères sur le développement des interactions mère-enfant et du nourrisson.

Topics: Acceptance and Commitment Therapy; Adolescent; Adult; Aged; Aldehydes; Alpinia; Animals; Antibodies, Monoclonal, Humanized; Antibodies, Protozoan; Anxiety; Apoptosis; Arabidopsis; Arabidopsis Proteins; ATP Binding Cassette Transporter, Subfamily B; bcl-2-Associated X Protein; Bile Duct Neoplasms; Bile Ducts, Intrahepatic; Brain-Derived Neurotrophic Factor; Calcitonin; Calcium Channels, N-Type; Capsaicin; Carbon Dioxide; Carcinoma, Neuroendocrine; Case-Control Studies; Cats; Cell Line, Tumor; Child; China; Cholangiocarcinoma; Cholesterol, HDL; Chromatography, High Pressure Liquid; Chromatography, Liquid; Circadian Clocks; Circadian Rhythm; Coculture Techniques; Cognition; COVID-19; Cross-Sectional Studies; Diagnostic Imaging; Dried Blood Spot Testing; Droughts; Dyslipidemias; East Asian People; Endocytosis; Ethanol; Female; Flavonoids; Ganglia, Spinal; Gastric Mucosa; Gene Expression Regulation, Plant; Genital Neoplasms, Female; Glucosinolates; Gold; Health Status; Humans; Hydrogen; Hypoparathyroidism; Immunoglobulin G; Immunoglobulin M; Immunosuppressive Agents; Individuality; Internet Addiction Disorder; Isopropyl Thiogalactoside; Lactams; Leg; Light; Lupus Erythematosus, Systemic; Lymph Node Excision; Male; Mammals; Mice; Microscopy; Middle Aged; Muscle Proteins; Muscle, Skeletal; Nanotubes; Neoplasms; NF-kappa B; Nuclear Receptor Subfamily 1, Group D, Member 1; Obesity; Outcome Assessment, Health Care; Ovarian Neoplasms; Pandemics; Parathyroid Glands; Philadelphia Chromosome; Polymorphism, Single Nucleotide; Posture; Powders; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Prednisone; Prevalence; Prognosis; Quadriceps Muscle; Quality of Life; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Retrospective Studies; Risk Factors; Saudi Arabia; Seroepidemiologic Studies; Spinal Cord; Stomach Ulcer; Students; Sulfhydryl Compounds; Surface Plasmon Resonance; Tandem Mass Spectrometry; Thyroid Cancer, Papillary; Thyroid Neoplasms; Thyroidectomy; Toll-Like Receptor 4; Toxoplasma; Toxoplasmosis; Transcriptome; Treatment Outcome; Triglycerides; TRPV Cation Channels; Tumor Microenvironment; Tumor Suppressor Protein p53; Ultrasonography, Interventional; Universities; Zebrafish

This study aims to determine the rates of clinically-significant anxiety and depressive symptoms during the immediate postpartum in a sample of women referred to a university maternity department, as well as the associated risk factors and the relations with the level of maternal bonding.. During the third national lockdown for the COVID-19 pandemic (February-April 2021), on days 2-3 after delivery 127 mothers were administrated the Edinburgh postnatal depression scale (EPDS), the state-trait anxiety inventory (STAI-YA), the mother-to-infant bonding scale (MIBS) and questions issued from the coronavirus health impact survey questionnaire (CRISIS).. The observed rates of anxiety and depressive symptoms were in the same range as those reported in observational studies conducted in high-resource countries during the COVID-19 pandemic. Risk of being infected by the SARS-CoV2 was both an independent risk factor for anxiety and depressive symptoms. The relations between the measure of maternal bonding and the severity of maternal emotional symptoms call for a better consideration of the long-term consequences of the pandemic on children's socio-emotional development.. Cette étude a pour objectif de déterminer la fréquence des symptômes dépressifs et anxieux maternels lors de la période du post-partum immédiat dans un groupe de femmes venant d’accoucher sur un service universitaire, ainsi que les facteurs de risques associés et les conséquences sur le lien mère-enfant.. Au tout début de la pandémie de COVID-19 (entre les mois de février et avril 2021), 127 femmes venant d’accoucher ont complété dans les 2 à 3 jours qui suivent l’accouchement l’Edinburgh postnatal depression scale (EPDS), le State-trait anxiety inventory (STAI-YA), le Mother-to-infant bonding scale (MIBS) et le Coronavirus health impact survey questionnaire (CRISIS).. La fréquence des symptômes dépressif cliniquement significatifs était de 17 % et de 15 % pour les symptômes anxieux. Le risque d’infection par le SARS-CoV2 était associé à la sévérité de ces symptômes dans les analyses multivariées. Des corrélations statistiquement significatives ont été mis en évidence entre le score MIBS et le score EPDS (rs = 0.26) et avec le score STAI-YA (rs = 0.26).. La fréquence des symptômes anxieux et dépressifs du péripartum étaient comparable avec d’autres études conduites dans les pays à haut niveau de ressource au cours de la pandémie de COVID-19. Le risque d’infection par le SARS-CoV2 est associé à des niveaux plus élevés de symptômes anxieux et dépressifs, à côté des autres facteurs connus de symptômes émotionnels du post-partum. Les liens retrouvés entre ces symptômes et le niveau de lien mère-enfant invitent à être attentif aux conséquences à long-terme de la pandémie sur le développement socio-émotionnel du nourrisson.. Des études complémentaires sont nécessaires pour confirmer ces résultats et déterminer les conséquences potentiellement délétères sur le développement des interactions mère-enfant et du nourrisson.

Topics: Acceptance and Commitment Therapy; Adolescent; Adult; Aged; Aldehydes; Alpinia; Animals; Antibodies, Monoclonal, Humanized; Antibodies, Protozoan; Anxiety; Apoptosis; Arabidopsis; Arabidopsis Proteins; ATP Binding Cassette Transporter, Subfamily B; bcl-2-Associated X Protein; Bile Duct Neoplasms; Bile Ducts, Intrahepatic; Brain-Derived Neurotrophic Factor; Calcitonin; Calcium Channels, N-Type; Capsaicin; Carbon Dioxide; Carcinoma, Neuroendocrine; Case-Control Studies; Cats; Cell Line, Tumor; Child; China; Cholangiocarcinoma; Cholesterol, HDL; Chromatography, High Pressure Liquid; Chromatography, Liquid; Circadian Clocks; Circadian Rhythm; Coculture Techniques; Cognition; COVID-19; Cross-Sectional Studies; Diagnostic Imaging; Dried Blood Spot Testing; Droughts; Dyslipidemias; East Asian People; Endocytosis; Ethanol; Female; Flavonoids; Ganglia, Spinal; Gastric Mucosa; Gene Expression Regulation, Plant; Genital Neoplasms, Female; Glucosinolates; Gold; Health Status; Humans; Hydrogen; Hypoparathyroidism; Immunoglobulin G; Immunoglobulin M; Immunosuppressive Agents; Individuality; Internet Addiction Disorder; Isopropyl Thiogalactoside; Lactams; Leg; Light; Lupus Erythematosus, Systemic; Lymph Node Excision; Male; Mammals; Mice; Microscopy; Middle Aged; Muscle Proteins; Muscle, Skeletal; Nanotubes; Neoplasms; NF-kappa B; Nuclear Receptor Subfamily 1, Group D, Member 1; Obesity; Outcome Assessment, Health Care; Ovarian Neoplasms; Pandemics; Parathyroid Glands; Philadelphia Chromosome; Polymorphism, Single Nucleotide; Posture; Powders; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Prednisone; Prevalence; Prognosis; Quadriceps Muscle; Quality of Life; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Retrospective Studies; Risk Factors; Saudi Arabia; Seroepidemiologic Studies; Spinal Cord; Stomach Ulcer; Students; Sulfhydryl Compounds; Surface Plasmon Resonance; Tandem Mass Spectrometry; Thyroid Cancer, Papillary; Thyroid Neoplasms; Thyroidectomy; Toll-Like Receptor 4; Toxoplasma; Toxoplasmosis; Transcriptome; Treatment Outcome; Triglycerides; TRPV Cation Channels; Tumor Microenvironment; Tumor Suppressor Protein p53; Ultrasonography, Interventional; Universities; Zebrafish

Toxoplasmosis, despite advances in science and technology, is a disease that requires attention since there is no vaccine capable of immunizing humans and animals against all isolated types of Toxoplasma gondii. Thus, the use of chimeric proteins, which can contain multiple antigens, is a very promising alternative for the process of obtaining a vaccine and diagnostic test for toxoplasmosis due to the great diversity of antigens presented by T. gondii. In this context, the present study evaluates batch culture strategies in the production of the multi-antigenic chimeric protein TgAGS/BsT from Toxoplasma gondii. Several exploratory cultures were initially carried out to observe the kinetic behavior of E. coli BL21 Star in five different medium compositions without the addition of IPTG (inducer). Cultures of E. coli B21 Star were carried out with 1.0 mM IPTG at different times of initiation of induction (0.5, 1, and 6 h) to evaluate the effects on cell growth, production of the protein of interest, culture pH, and acetic acid formation. The results showed that among the culture media evaluated, 2xTY and TB supplemented with glycerol had the best cell concentration values of 3.42 ± 0.05 g/L and 5.48 ± 0.05 g/L, respectively. In the assays induced by IPTG, a higher expression of TgAGS/BsT protein was observed, with induction beginning within 6 h of culture, with a maximum concentration of protein of interest of 1.82 ± 0.02 g/L for the 2xTY and 2.49 ± 0.03 g/L for the TB medium. In addition, later induction by IPTG provided greater stability of plasmid pET-TgAGS, remaining with values above 90% at the end of culture.

Topics: Animals; Antigens, Protozoan; Culture Media; Escherichia coli; Escherichia coli Infections; Glycerol; Humans; Isopropyl Thiogalactoside; Protozoan Proteins; Recombinant Fusion Proteins; Recombinant Proteins; Toxoplasma; Toxoplasmosis