isopropyl-thiogalactoside and Liver-Neoplasms

Hepatocellular carcinoma (HCC) is one of the prevalent cancers in the world with a high recurrence rate. In recent years, different researches have focused on designing efficient multi-epitope peptide vaccines against HCC. In designing these vaccines, over-expressed antigens in HCC patients, such as α- fetoprotein (AFP) and glypican-3 (GPC-3), have been employed. In our previous study, a multi-epitope peptide vaccine for HCC was designed by. This research is experimental and was carried out in Fasa, Iran, in 2017. The designed vaccine construct gene was transformed to the. The best conditions for protein expression were obtained in the Super Optimal Broth (SOB) medium at 37 °C after the induction of expression by 1 mM IPTG for six hour.. The recombinant HCC vaccine was produced with a proper concentration.

Topics: alpha-Fetoproteins; Carcinoma, Hepatocellular; Epitopes; Humans; Isopropyl Thiogalactoside; Liver Neoplasms; Vaccines, Subunit