isocoumarin-nm-3 and Disease-Models--Animal

To explore the molecular mechanisms of action of paclitaxel and NM-3 on human gastric cancer in severe combined immune deficiency (SCID) mice.. Human gastric cancer cells SGC-7901 were implanted into SCID mice and mice were treated with paclitaxel and NM-3. The effects of paclitaxel and NM-3 on apoptosis of human gastric cancer cells were analyzed using flow cytometry, TUNEL assays, and DNA fragment analyses.. Apoptosis of SGC-7901 cells was successfully induced by paclitaxel, NM-3, and the combination of paclitaxel and NM-3 24 h after injection as shown by the presence of apoptotic hypodiploid peaks on the flow cytometer before G1-S and a characteristic apoptotic band pattern in the DNA electrophoresis. The apoptotic rate detected by TUNEL assay was found to be significantly higher in the paclitaxel/NM-3 compared to the control group (38.5% +/- 5.14% vs 13.2% +/- 1.75%, P < 0.01).. Paclitaxel in combination with NM-3 is able to induce apoptosis of the human gastric cancer cells in SCID mice effectively and synergistically.

Topics: Adenocarcinoma; Animals; Antigens, Viral, Tumor; Antineoplastic Agents; Apoptosis; Cell Line, Tumor; Disease Models, Animal; Drug Synergism; Drug Therapy, Combination; Humans; Isocoumarins; Male; Mice; Mice, Nude; Mice, SCID; Paclitaxel; Stomach Neoplasms; Viral Core Proteins; Xenograft Model Antitumor Assays