iridoids and Periodontitis

The present study aimed to evaluate the effects of oleuropein on ligature-induced alveolar bone loss. In this respect, osteoblastic activity, osteoclastic activity, inflammatory markers, and apoptosis were evaluated.. Oleuropein is a flavonoid, which has potent anti-inflammatory and bone-protective effects.. Thirty-two Wistar rats were divided into four experimental groups as following: control (C, n = 8) group; periodontitis (P, n = 8) group; periodontitis and low-dose oleuropein group (12 mg/kg/day oleuropein, LDO group, n = 8); and periodontitis and high-dose oleuropein group (24 mg/kg/day oleuropein, HDO group, n = 8). Periodontitis was induced via ligatures. Study period was 14 days, and animals were sacrificed at end of this period. Mandibles were examined via a stereomicroscope and underwent histological procedures. Osteoblast, tartrate-resistant acid phosphatase (TRAP)-positive osteoclast, and inflammatory cell counts were determined in hematoxylin-eosin stained sections. Inducible nitric oxide synthase (iNOS), bone morphogenetic protein-4, the cluster of differentiation (CD)-68, cysteine-aspartic proteases-3 (Caspase 3), and B-cell lymphoma-2 (Bcl-2) expressions were evaluated via immunohistochemistry.. Periodontitis group had highest alveolar bone loss, and these levels significantly decreased in LDO and HDO groups. Both 12 and 24 mg/kg oleuropein groups significantly increased osteoblast cell counts and decreased TRAP-positive osteoclast and inflammatory cell counts. BMP-4 and bcl-2 expressions were elevated in oleuropein groups while caspase-3 expressions decreased. iNOS and CD68 were higher in periodontitis group compared to control group, but there was no significant difference between other groups.. Oleuropein successfully decreased alveolar bone loss as a result of decreased osteoclastic activity, inflammation, and apoptosis and increased osteoblastic activity.

Topics: Alveolar Bone Loss; Animals; Antigens, CD; Antigens, Differentiation, Myelomonocytic; Apoptosis; Bone Morphogenetic Protein 4; Caspase 3; Female; Inflammation; Iridoid Glucosides; Iridoids; Nitric Oxide Synthase Type II; Osteoblasts; Osteoclasts; Periodontitis; Proto-Oncogene Proteins c-bcl-2; Rats; Rats, Wistar; Tartrate-Resistant Acid Phosphatase

The aim of the study was the evaluation of the action of a new natural drug--Naran S, elaborated in the Department of Inorganic Chemistry of the Medical University of Lublin (patent registration P.332066) in the treatment of inflammatory conditions of the oral cavity mucous membrane. The experiment was conducted on male Wistar rats. Topical inflammation of the gum was induced by injecting a complete Freund adjuvant (AF) (Calbiochem) into the inter-dental papilla of the lower incisors. The material was taken, fixed and stained with hematoxylin and eosin and by the use of the Masson's method after 24 hours, 1, 2, and 4 weeks following the injection time. Morphology of the mucous membrane was examined and it was noticed that in the group treated with Naran S infusion the complete rebuilding of the epithelial strata, collagen fibres took place. The shape of the cells was regular and there occurred the elimination of the tissue fluid in the proper mucous membrane. The results obtained in the study allow to claim that the topically given plant drug thanks to its contents of biologically active substance such as: iridoids, flavonoids, phenol acids, tannins, displays curative activity in the therapy of the inflammatory conditions of the oral cavity mucous membrane.

Topics: Animals; Anti-Inflammatory Agents; Disease Models, Animal; Flavonoids; Freund's Adjuvant; Gingiva; Gingivitis; Iridoids; Male; Mouth Mucosa; Mouthwashes; Periodontitis; Periodontium; Phenols; Phytotherapy; Plant Extracts; Rats; Rats, Wistar; Tannins; Treatment Outcome