iridoids and Hepatitis-B

Three new secoiridoids, swermacrolactones A-C (1-3), together with fourteen known compounds were isolated from Swertia macrosperma. Their structures were elucidated based on extensive spectroscopic analyses (IR, UV, MS, 1D and 2D NMR). By anti-HBV assay on the Hep G 2.2.15 cell line in vitro, the most active compound, luteolin (9), inhibited the secretion of hepatitis B virus surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg) with IC50 values of 0.02 and 0.02 mM, respectively.

Topics: Antigens, Viral; Antiviral Agents; Hep G2 Cells; Hepatitis B; Hepatitis B virus; Humans; Inhibitory Concentration 50; Iridoids; Luteolin; Molecular Structure; Phytotherapy; Plant Extracts; Swertia

Jasminum officinale L. var. grandiflorum (JOG) is a folk medicine used for the treatment of hepatitis in south of China. Phytochemical studies showed that secoiridoid glycosides are the typical constituents of this plant.. The present study was undertaken to evaluate the effect of oleuropein (Ole) derived from the flowers of JOG on hepatitis B virus (HBV) replication in HepG2 2.2.15 cell line in vitro and duck hepatitis B virus (DHBV) replication in ducklings in vivo.. The extracellular hepatitis B e antigen (HBeAg) and hepatitis B surface antigen (HBsAg) concentrations in cell culture medium were determined by ELISA. DHBV in duck serum was analyzed by dot blot.. Ole blocks effectively HBsAg secretion in HepG2 2.2.15 cells in a dose-dependent manner (IC(50)=23.2 microg/ml). Ole (80 mg/kg, intraperitoneally, twice daily) also reduced viremia in DHBV-infected ducks.. Ole therefore warrants further investigation as a potential therapeutic agent for HBV infection.

Topics: Animals; Antiviral Agents; Cell Line; Disease Models, Animal; Dose-Response Relationship, Drug; Ducks; Flowers; Hepatitis B; Hepatitis B Surface Antigens; Hepatitis B virus; Hepatitis B Virus, Duck; Hepatitis, Viral, Animal; Humans; Iridoid Glucosides; Iridoids; Jasminum; Phytotherapy; Plant Extracts; Pyrans; Viremia; Virus Replication