intrinsic-factor and Diarrhea

To make a food preparation containing radioactively labelled cobalamins, rabbits were given repeated injections with 57Co-labelled cyanocobalamin. The liver was removed, homogenized, and fried for 1 min or boiled for 30 min. Of the radioactivity in the fried homogenate 41.7% was recovered in the centrifuged supernatant compared with 50.8% in the boiled homogenate. The radioactivity in the supernatants had a molecular size close to that of free 57Co-labelled cyanocobalamin. Forty-two per cent of the radioactivity in the whole homogenate had been incorporated into 5-deoxyadenosyl-, 10% into methyl-, and 16.5% into hydroxy-cobalamin. To assess the validity of a double-isotope method for measuring the intestinal absorption of doses of the 57Co-labelled liver cobalamins, 51CrCl3 was used as a non-absorbable marker. In 14 healthy volunteers the correlation coefficient between the absorption measured by the double-isotope technique and the faecal excretion test was highly significant (r = 0.96, p less than 0.005), and there was only a small variation in the 57Co/51Cr ratio in successive stool collections. In 11 patients with chronic diarrhoea there was a significant correlation between the absorption measured by the double-isotope technique and the faecal excretion test (r = 0.92, p less than 0.005), but in some patients there was considerable variation in the 57Co/51Cr ratio in successive stool collections.

Topics: Adult; Aged; Animals; Chromatography, Gel; Chronic Disease; Cobalt Radioisotopes; Diagnosis, Differential; Diarrhea; Food; Humans; Intestinal Absorption; Intrinsic Factor; Liver; Middle Aged; Molecular Weight; Rabbits; Transcobalamins; Vitamin B 12

Topics: Afferent Loop Syndrome; Anemia, Macrocytic; Diarrhea; Dumping Syndrome; Folic Acid Deficiency; Gastrectomy; Gastric Juice; Gastrins; Humans; Intestinal Absorption; Intrinsic Factor; Mucus; Osteoporosis; Postgastrectomy Syndromes; Stomach Neoplasms; Vitamin B 12 Deficiency; Vomiting

Topics: Body Height; Child; Child Nutritional Physiological Phenomena; Child, Preschool; Cobalt Isotopes; Convalescence; Creatinine; Diarrhea; Diarrhea, Infantile; Diet Therapy; Dietary Proteins; Humans; Ileum; Infant; Infant Nutrition Disorders; Infant Nutritional Physiological Phenomena; Intrinsic Factor; Malabsorption Syndromes; Nutrition Disorders; Protein-Energy Malnutrition; Schilling Test; Vitamin B 12

Topics: Adult; Aged; Diarrhea; Enteritis; Feces; Female; Gastrectomy; Gastric Acidity Determination; Gastric Juice; Gastric Mucosa; Humans; Intrinsic Factor; Male; Middle Aged; Radioimmunoassay; Salmonella; Salmonella Infections; Salmonella paratyphi A; Salmonella typhimurium; Time Factors

Topics: Absorption; Adult; Cholera; Chromium Isotopes; Cobalt Isotopes; Diarrhea; Diffusion; Edetic Acid; Glomerular Filtration Rate; Humans; Ileum; India; Indicator Dilution Techniques; Indicators and Reagents; Intestinal Absorption; Intestine, Small; Intrinsic Factor; Intubation, Gastrointestinal; Jejunum; Male; Sulfobromophthalein; Vitamin B 12; Xylose

Topics: Biguanides; Cobalt Isotopes; Diabetes Mellitus; Diarrhea; Humans; Intestinal Absorption; Intrinsic Factor; Obesity; Schilling Test; Vitamin B 12

Topics: Achlorhydria; Adult; Agammaglobulinemia; Anemia, Pernicious; Antibodies; Arthritis, Rheumatoid; Atrophy; Autoimmune Diseases; Colitis, Ulcerative; Diarrhea; Female; Fluorescent Antibody Technique; gamma-Globulins; Gastric Mucosa; Gastritis; Giardiasis; Humans; Hypersensitivity; Hypersensitivity, Delayed; Infections; Intrinsic Factor; Male; Middle Aged; Vitamin B 12

Topics: Adult; Anemia, Pernicious; Antibodies; Blood Protein Disorders; Blood Proteins; Cobalt Isotopes; Diarrhea; gamma-Globulins; Humans; Intrinsic Factor; Male; Vitamin B 12

Topics: Cobalt Isotopes; Corrinoids; Diarrhea; Digestion; Humans; Intrinsic Factor; Pellagra; Vitamin B 12