interleukin-8 and Wounds--Gunshot

Increased pulmonary vascular permeability is a hallmark of acute lung injury (ALI). Gap junction channels (GJCs) connect adjacent cells and facilitate ion exchange. It remained unclear whether GJCs modulate pulmonary permeability in ALI through intracellular calcium.. This study aimed to verify if GJCs in pulmonary microvessel endothelial cells (PMVECs) modulate pulmonary vascular permeability in ALI via intracellular calcium.. Firstly, an animal model of ALI was studied using connexin 40 (Cx40) immunohistochemistry in the lung with Evans' blue (EB) leakage. Then cultured PMVECs were divided into three groups: G(control), G(serum) and G(blocker). Serum was obtained from animals with ALI following gunshot injury (injured serum). Initially, G(blocker) was treated with the blocker of GJCs, and then G(serum) and G(blocker) were stimulated with the injured serum, respectively. GJCs, the permeability of cell monolayers and intracellular Ca(2+) were assessed.. Cx40 time-dependently decreased, whereas EB leakage increased. Cx40 and EB leakage exhibited a strong inverse correlation (rho = -0.934, p < 0.05). Injured serum decreased GJCs and expression of Cx40, whereas the blocker aggravated this effect. Similarly, when PMVEC monolayer was treated with injured serum, both permeability and intracellular Ca(2+) increased. These effects were also aggravated with the blocker.. Depression of GJCs of PMVECs increased pulmonary vascular permeability in ALI; this effect may be mediated by the overload of intracellular calcium.

Topics: Acute Lung Injury; Animals; Blotting, Western; Calcium; Capillary Permeability; Cells, Cultured; Coloring Agents; Connexins; Endothelial Cells; Evans Blue; Gap Junction alpha-5 Protein; Gap Junctions; Heptanol; Interleukin-8; Intracellular Fluid; Lung; Rabbits; Tumor Necrosis Factor-alpha; Wounds, Gunshot

To study the relationship between proinflammentory cytokines and wound healing in local area.. Interleukin(IL)-6, IL-8 and tumor necrosis factor-alpha (TNF-alpha) expression in local soft tissues after lower jaw gunshot wound were assessed by using immunohisto-chemistry, and pathological methods.. High expressions of these cytokines were observed in local soft tissues of wounds after the gunshot. The cytokines expressions were different between the area near the bullet path and that far from the path. There was only one peak of the cytokines concentrations in the area far from the bullet path, while there were two peaks of the cytokines concentrations in the area near the bullet path, specially IL-6, IL-8. There were time differences for some different cytokines to reach the peaks of the their concentrations. In wound situ, the fibroblasts, mononuclear phagocytes and endothelial cells expressed IL-6, IL-8 and TNF-alpha after the firearm.. These results suggest that proinflammatory cytokines play an important role in regulating inflammatory cytokine reactions of local injury tissues. They might improve the functions of fibroblasts, mononuclear phagocytes and endothelial cells and stimulate wound cells to produce and release other cytokines.

Topics: Animals; Cytokines; Dogs; Immunohistochemistry; Interleukin-6; Interleukin-8; Maxillofacial Injuries; Time Factors; Tumor Necrosis Factor-alpha; Wounds, Gunshot