interleukin-8 and Urinary-Retention

Acute urinary retention (AUR) is one of the progressive manifestations of benign prostatic hyperplasia (BPH). This cross-sectional study was conducted to analyse the possible association between serum interleukin 8 (sIL-8) and AUR in BPH patients to provide evidence of sIL-8 as a potential biomarker for the prediction of AUR. The relationship between sIL-8 levels and AUR was evaluated by logistic regressions in 245 ageing Chinese men with BPH. The discriminant validity of sIL-8 and the optimal cut-off value were determined by a receiver operating characteristic curve. The levels of sIL-8 increased significantly in BPH patients with AUR (p < 0.001). The sIL-8 concentration was positively correlated with AUR in BPH patients (OR = 1.024, 95% CI: 1.009-1.040, p = 0.002). The correlation with AUR in the group with a high sIL-8 level (≥43.05 pg/ml) was significantly enhanced (OR = 8.853, 95% CI: 2.433-32.205, p = 0.001). The sIL-8 level correlated with AUR in Chinese BPH patients independently. As a possible predictor, sIL-8 may contribute to the screening of high-risk populations for AUR to create opportunities for the early effective interventions to improve prognosis and enhance the quality of life. Prospective studies are needed to support all these results.

Topics: China; Cross-Sectional Studies; Humans; Interleukin-8; Male; Prospective Studies; Prostatic Hyperplasia; Quality of Life; Urinary Retention

To investigate the expressions of interleukin-17 (IL-17) and interleukin-8 (IL-8) in benign prostatic hyperplasia (BPH) and BPH complicated with histological inflammation and their significance.. According to the results of HE staining, we divided 60 cases of BPH treated by transurethral resection of the prostate (TURP) into a BPH group (n = 23) and a BPH with inflammation group (n = 37). We analyzed the clinical data of the patients and determined the mRNA and protein expressions of IL-17 and IL-8 by immunohistochemistry, real-time fluorescence quantitative PCR, and Western blot, respectively.. Compared with the BPH patients complicated with inflammation, the BPH group showed significantly lower International Prostate Symptom Score (IPSS) (29.1 ± 6.2 vs 21.6 ± 3.7), quality of life score (QoL) (5.4 ± 1.3 vs 4.4 ± 1.6), postvoid residual urine volume (RUV) (［198.6 ± 15.5］ vs ［98.2 ± 19.3］ ml), prostate volume (［69.2 ± 24.1］ vs ［49.8 ± 16.5］ ml), PSA level (［7.4 ± 1.9］ vs ［2.8 ± 0.8］ μg/L) and serum c-reactive protein content (CRP) (［5.1±2.0］ vs ［1.5±0.6］ mg/L), but a higher maximum urine flow rate (Qmax) (［4.7 ± 2.1］ vs ［8.2 ± 1.8］ ml/s) (all P<0.05). The former group had a significantly higher incidence rate of urinary retention than the latter (32.4% ［12/37］ vs 8.69% ［2/23］), mRNA expressions of IL-17 (0.303 ± 0.076 vs 0.042 ± 0.019) and IL-8 (0.536 ± 0.059 vs 0.108 ± 0.025), and protein expressions of IL-17 (0.88 ± 0.10 vs 0.34 ± 0.05) and IL-8 (1.07 ± 0.08 vs 0.43 ± 0.04) (all P<0.05).. The expressions of IL-17 and IL-8 are upregulated in the prostatic tissue of the BPH patients with inflammation, which may play a significant role in the development and progression of BPH.. 目的： 探讨单纯良性前列腺增生（BPH）与伴有前列腺炎的BPH患者前列腺组织中IL-17和 IL-8表达差异。方法： 收集福建医科大学附属第一医院行经尿道前列腺电切术的60例BPH患者标本。根据标本HE染色不同分为单纯BPH组23例和合并炎症的BPH组37例。对两组患者的临床资料进行分析比较；用免疫组织化学方法检测各标本IL-17和IL-8的表达情况，用荧光定量PCR和Western印迹检测各标本IL-17和IL-8 mRNA和蛋白的表达。结果： 单纯BPH组和合并炎症的BPH组患者的国际前列腺症状评分（IPSS）［（21.6±3.7）分 vs （29.1±6.2）分］、生活质量评分（QoL）［（4.4±1.6）分 vs (5.4±1.3) 分］、最大尿流率（Qmax）［(8.2±1.8) ml/s vs (4.7±2.1) ml/s］、残余尿量（RUV）［(98.2±19.3) ml vs (198.6±15.5) ml］、前列腺体积［(49.8±16.5) ml vs (69.2±24.1) ml］、PSA［(2.8±0.8) μg/L vs (7.4±1.9) μg/L］和C反应蛋白（CRP）［(1.5±0.6) mg/L vs (5.1±2.0) mg/L］,两组相比差异明显，有统计学意义（P<0.05）；合并炎症的BPH组患者尿潴留的发生率［32.4%（12/37）］明显高于单纯BPH组［8.69%（2/23），P<0.05］；IL-17 mRNA和IL-8 mRNA在合并炎症的BPH组前列腺组织中的表达量(0.303±0.076和0.536±0.059)均明显高于单纯BPH组（0.042±0.019和0.108±0.025，P<0.05）；IL-17和IL-8蛋白在单纯BPH组和在合并炎症的BPH组患者前列腺组织中的蛋白表达(0.34±0.05 vs 0.88±0.10、0.43±0.04 vs 1.07±0.08)，两者相比差异显著，有统计学意义（P<0.05）。结论：IL-17和IL-8在合并炎症的BPH患者前列腺组织中的表达上调，其可能在BPH的发生发展中起重要作用。.

Topics: Disease Progression; Humans; Inflammation; Interleukin-17; Interleukin-8; Male; Organ Size; Prostate; Prostatic Hyperplasia; Quality of Life; RNA, Messenger; Transurethral Resection of Prostate; Treatment Outcome; Urinary Retention