interleukin-8 and Tinea

Trichophyton mentagrophytes (T. mentagrophytes) is the main cause of rabbit dermatophytosis. As the main pathogen-associated molecular pattern of T. mentagrophytes, the role of β-glucan in the pathogenesis of rabbit dermatophytosis remains elusive. Keratinocytes (KC) are the main cellular component and the first defensive line against fungal pathogens in the skin. Therefore, the present study investigated the effects of β-glucan on rabbit KC from dorsal skin. β-glucan was found to inhibit KC proliferation by 10% at 20 ug/ml and this concentration was thus considered as optimal. Next, 20 ug/ml β-glucan stimulation for 24 h significantly increased CXCL8, CXCL11, and IL-1β secretions in KC. Furthermore, β-glucan exposure induced the expressions of JAK2 mRNA, STAT3 mRNA, and p-STAT3 protein. Silencing JAK2 expression inhibited p-STAT3 protein expression and β-glucan-induced IL-1β secretion. And overexpression of JAK2 further promoted β-glucan-mediated p-STAT3 protein and IL-1β productions. These results suggested that β-glucan-induced CXCL8, CXCL11, and IL-1β secretions in rabbit KC might be involved in the inflammatory response of T. mentagrophytes infected rabbit dorsal skin. However, only IL-1β secretion was promoted by the JAK2/STAT3 signaling pathway. In conclusion, this study is a necessary step toward elucidating the mechanisms that underlie skin immune system injury stimulated by β-glucan.

Topics: Animals; Arthrodermataceae; beta-Glucans; Cells, Cultured; Chemokine CXCL11; Interleukin-1beta; Interleukin-8; Janus Kinase 2; Keratinocytes; Rabbits; Signal Transduction; STAT3 Transcription Factor; Tinea

Dermatophytosis (tinea) is a common disease in superficial mycoses and is generally confined to the stratum corneum in the epidermis and cutaneous appendages. The mechanisms by which dermatophytes cause dermatophytosis, however, are poorly understood. In this study, we evaluated the effect of Trichophyton mentagrophytes, T. tonsurans and T. rubrum on cytokine production by normal human epidermal keratinocytes (NHEKs). After 3-24 h of co-culture of NHEKs with each of the dermatophytes, cytokines in the supernatant were measured by enzyme-linked immunosorbent assay. Promoter activity of IL-8 was measured by chloramphenicol acetyl transferase (CAT) assay. IL-8 and GRO-alpha levels were higher in supernatants co-cultured with T. mentagrophytes isolates from animal than in those with T. mentagrophytes isolates from human, and with T. tonsurans and T. rubrum isolates. CAT expression for IL-8 promoter activity was higher in cell lysates stimulated with T. mentagrophytes isolates from animal than in those with T. mentagrophytes isolates from human, and with T. tonsurans and T. rubrum isolates. These findings suggest that dermatophytes directly induce production of cytokines at the transcriptional level by human keratinocytes, and that there are differences in their ability to induce cytokine production between the dermatophytes.

Topics: Cell Survival; Cells, Cultured; Chemokine CCL2; Chemokine CCL5; Chemokine CXCL1; Enzyme-Linked Immunosorbent Assay; Epidermal Cells; Epidermis; Granulocyte-Macrophage Colony-Stimulating Factor; Humans; Interleukin-1beta; Interleukin-6; Interleukin-8; Keratinocytes; Microbiological Techniques; Promoter Regions, Genetic; Tinea; Trichophyton; Tumor Necrosis Factor-alpha

The enzymic activity of Trichophyton rubrum has been investigated in relation to the plasma-dependent chemotaxis of polymorphonuclear leucocytes (PMNs). In Boyden-type experiments use of a cytoplasmic extract of T. rubrum (CETr) produces neutrophil chemotactic factor (NCF) from plasma. CETr was shown to have activity for eight enzymes: heat treatment of CETr led to a partial loss of activity for seven enzymes and a significant reduction in the number of PMNs migrating. Addition of CETr to plasma and incubation for 18 h at 37 degrees C before use led to complete loss of chemotactic activity. The similar incubation of plasma with trypsin led to a complete loss of chemotactic activity. CETr has greater activity than trypsin in the production of NCF from plasma. The results are discussed in relation to reports on the importance of serine esterases in PMN chemotaxis. The failure of PMNs to migrate into keratinized tissue infected with T. rubrum is noted and it is suggested that the high enzymic activities necessary for the colonization of keratinized tissue effect a breakdown of NCF.

Topics: Animals; Cell Migration Inhibition; Chemotactic Factors; Chemotaxis, Leukocyte; Complement System Proteins; Humans; Interleukin-8; Neutrophils; Rabbits; Skin; Temperature; Tinea; Trichophyton