interleukin-8 and Rupture

This cross-sectional clinical study compared inflammation, including expression of the chemokine interleukin (IL)-8 and intercellular cell adhesion molecule-1 (ICAM-1), in the stifle joints of 4 control dogs and 23 dogs with cranial cruciate ligament rupture (CCLR). The CCL, synovial membrane, meniscus, cartilage, and synovial fluid from the affected stifle joints of all the dogs were examined. Inflammatory cell counts were performed on the synovial fluid, and the tissues were processed for histologic study and immunohistochemical detection of IL-8 and ICAM-1. The synovial fluid from the stifle joints of the dogs with CCLR had an increased percentage of neutrophils (P = 0.054) and a decreased percentage of lymphocytes (P = 0.004) but not macrophages compared with the fluid from the control dogs. There was accumulation of inflammatory cells and increased expression of IL-8 and ICAM-1 in the vascular endothelium of the synovial membrane and the CCL of the dogs with CCLR. The increase in inflammatory cells in the stifle joints of dogs with CCLR may therefore be due to increased expression of IL-8 and ICAM-1 in the synovial membrane and the CCL after the injury. These data may help in understanding the mechanisms of inflammation associated with CCLR.

Topics: Animals; Anterior Cruciate Ligament; Case-Control Studies; Dog Diseases; Dogs; Immunohistochemistry; Intercellular Adhesion Molecule-1; Interleukin-8; Leukocyte Count; Rupture; Stifle; Synovial Fluid; Synovial Membrane

To score the number of plaque neutrophils and relate the score to plaque morphology and inflammatory status.. Neutrophils are inflammatory cells with tissue destruction capabilities that have been found at the site of an atherosclerotic plaque rupture or erosion. Poor evidence exists for neutrophil infiltration in human carotid atherosclerotic plaques, and its association with plaque morphology has not yet been described. A set of 355 human carotid plaques was stained for the neutrophil marker CD66b. High neutrophil numbers were found in plaques with a large lipid core, high macrophage numbers, and low collagen amount and smooth muscle cell numbers. High neutrophil numbers were associated with high interleukin 8 (P<0.001) and matrix metalloproteases 8 (P=0.005) and 9 (P<0.001) plaque levels. High microvessel density within plaques was correlated with high neutrophil numbers (P=0.01). In addition, low numbers of neutrophils were associated with female sex and use of beta-blockers.. For the first time to our knowledge, these results show that neutrophil numbers are strongly associated with the histopathologic features of rupture-prone atherosclerotic lesions and suggest a role for neutrophils in plaque destabilization.

Topics: Adrenergic beta-Antagonists; Aged; Aged, 80 and over; Antigens, CD; Biomarkers; Carotid Arteries; Carotid Artery Diseases; Cell Adhesion Molecules; Collagen; Disease Progression; Female; GPI-Linked Proteins; Humans; Immunohistochemistry; Interleukin-8; Leukocyte Count; Lipids; Logistic Models; Macrophages; Male; Matrix Metalloproteinase 8; Matrix Metalloproteinase 9; Microvessels; Middle Aged; Myocytes, Smooth Muscle; Netherlands; Neutrophil Infiltration; Neutrophils; Odds Ratio; Risk Assessment; Risk Factors; Rupture; Severity of Illness Index; Sex Factors; Up-Regulation