interleukin-8 and Retinal-Vein-Occlusion

Topics: Angiogenesis Inhibitors; Cytokines; Female; Humans; Interleukin-6; Interleukin-8; Macular Edema; Microcirculation; Placenta Growth Factor; Ranibizumab; Retinal Vein Occlusion; Tomography, Optical Coherence; Vascular Endothelial Growth Factor A

The mechanisms of neuronal degeneration and associated acute alterations in intraretinal cytokine and protein levels remain poorly understood in variable ischaemic retinopathies such as in branch retinal vein occlusion (BRVO). Herein we investigate neuronal damage and alterations in retinal cytokines and proteins in a pig model of acute BRVO. Twelve pigs had a BRVO induced photothrombotically in both eyes. Three pigs (6 eyes) each at 2, 6, 10 and 20 days were sacrificed together with an additional 3 control (6 eyes), enucleated, retinas dissected and processed. Apoptosis in the inner retina was determined by terminal deoxyynuclotidyl transferase mediated dUTP nick end labelling (TUNEL) and histology. Expression of glial acidic fibrillary protein (GFAP), aquaporin-4 (AQP4), inward rectifier potassium channel 10 protein (K

Topics: Animals; Aquaporin 4; Chemokine CXCL12; Cytokines; Disease Models, Animal; Eye Proteins; Glial Fibrillary Acidic Protein; Interleukin-6; Interleukin-8; Neurodegenerative Diseases; Potassium Channels, Inwardly Rectifying; Retinal Neurons; Retinal Vein Occlusion; RNA, Messenger; Swine; Vascular Endothelial Growth Factor A

To investigate the association of intraocular concentrations of inflammatory factors and improvement of macular edema after vitrectomy for patients with macular edema in branch retinal vein occlusion (BRVO).. Retrospective case-control study.. Seventeen patients with BRVO who underwent vitreous surgery for macular edema and 15 control patients were enrolled from Hachioji Medical Center of Tokyo Medical University. The concentrations of eight inflammatory factors were measured in vitreous and aqueous fluids obtained at the time of vitrectomy using a flow cytometer. Macular thickness was measured by optical coherence tomography before and one, three, and six months after surgery. Correlations between the concentrations of inflammatory factors and macular thickness were statistically analyzed.. Higher aqueous and vitreous concentrations of vascular endothelial growth factor (VEGF) and interleukin (IL)-8 were significantly correlated with a greater difference in macular thickness between before and six months after surgery (vitreous VEGF, P=.047; aqueous VEGF, P=.032; vitreous IL-8, P=.016; and aqueous IL-8, P=.032). Higher intraocular concentrations of monokine induced by interferon γ (Mig) were significantly correlated with a smaller degree of macular thickness six months after surgery (vitreous Mig, P=.038; aqueous Mig, P=.009).. High preoperative VEGF, IL-8, and Mig concentrations were associated with improvement of macular edema six months after vitreous surgery in patients with macular edema attributable to BRVO.

Topics: Aged; Aqueous Humor; Case-Control Studies; Chemokine CXCL9; Female; Flow Cytometry; Humans; Inflammation Mediators; Interleukin-8; Macula Lutea; Macular Edema; Male; Retinal Vein Occlusion; Retrospective Studies; Tomography, Optical Coherence; Vascular Endothelial Growth Factor A; Vitrectomy; Vitreous Body

To investigate whether interleukine-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) are related with macular oedema in patients with branch retinal vein occlusions (BRVOs).. Retrospective case-control study.. Nineteen patients who had macular oedema due to BRVO and nine patients with non-ischaemic ocular diseases (control group).. Macular oedema was examined by optical coherence tomography. Both venous blood and vitreous samples were obtained at the time of vitreoretinal surgery. IL-8 and MCP-1 levels in vitreous fluid and plasma were determined with enzyme-linked immunosorbent assay kits. Variables were compared with the Mann-Whitney U-test, Wilcoxon's signed-ranked test, and the chi2-test, when appropriate. To examine correlations, Spearman's rank-order correlation coefficients were calculated. Statistical significance was set at P<0.05.. The vitreous fluid levels of IL-8 (median: 63.5 pg/ml) and MCP-1 (median: 1522.4 pg/ml) were significantly higher in the patients with BRVO than in the control group (median: 5.1 and 746.5 pg/ml respectively; P<0.001 and <0.001 respectively). Vitreous IL-8 and MCP-1 were significantly correlated in patients with BRVO (P=0.009).. Both IL-8 and MCP-1 were elevated in the vitreous fluid of patients with BRVO and macular oedema. Both chemokines may contribute to the pathogenesis of macular oedema in patients with BRVO.

Topics: Aged; Biomarkers; Case-Control Studies; Chemokine CXCL2; Enzyme-Linked Immunosorbent Assay; Female; Humans; Interleukin-8; Macular Edema; Male; Middle Aged; Retinal Vein Occlusion; Retrospective Studies; Tomography, Optical Coherence; Vitreous Body