interleukin-8 and Myopia

The increased global incidence of myopia requires the establishment of therapeutic approaches. This study aimed to investigate the effect of Fallopia Japonica (FJ) and Prunella vulgaris (PV) extract on myopia caused by monocular form deprivation (MFD).. We used human retinal pigment epithelial cell to study the molecular mechanisms on how FJ extract (FJE) and PV extract (PVE) lowering the inflammation of the eye. The effect of FJE and PVE in MFD induced hamster model and explore the role of inflammation cytokines in myopia.. FJE + PVE reduced IL-6, IL-8, and TNF-α expression in RPE cells. Furthermore, FJE and PVE inhibited inflammation by attenuating the phosphorylation of protein kinase B (AKT), and nuclear factor kappa-light-chain-enhancer of activated B (NF-κB) pathway. In addition, we report two resveratrol + ursolic acid compounds from FJ and PV and their inhibitory activities against IL-6, IL-8, and TNF-α expression levels in RPE cells treated with IL-6 and TNF-α. FJE, PVE, and FJE + PVE were applied to MFD hamsters and their axial length was measured after 21 days. The axial length showed statistically significant differences between phosphate-buffered saline- and FJE-, PVE-, and FJE + PVE-treated MFD eyes. FJE + PVE suppressed expressions of IL-6, IL-8, and TNF-α. They also inhibited myopia-related transforming growth factor-beta (TGF)-β1, matrix metalloproteinase (MMP)-2, and NF-κB expression while increasing type I collagen expression.. Overall, these results suggest that FJE + PVE may have a therapeutic effect on myopia and be used as a potential treatment option.

Topics: Animals; Collagen Type I; Cricetinae; Fallopia japonica; Humans; Inflammation; Interleukin-6; Interleukin-8; Matrix Metalloproteinases; Myopia; NF-kappa B; Phosphates; Plant Extracts; Proto-Oncogene Proteins c-akt; Prunella; Resveratrol; Retinal Pigments; Transforming Growth Factors; Tumor Necrosis Factor-alpha

To study changes in tear film inflammatory mediators following continuous wear of silicone-hydrogel lenses and corneal refractive therapy with reverse geometry contact lenses.. A prospective, case-control study.. Twenty-eight subjects had worn silicone-hydrogel lenses on a 30-night continuous wear basis. Thirty-two subjects had worn corneal refractive therapy lenses on an overnight basis. Thirty-two matched control subjects were also recruited. Tear samples were obtained 12 months after initial fitting and assayed using ELISA for cytokines (interleukin (IL)-6 and IL-8), matrix metalloproteinase 9 (MMP-9) and epidermal growth factor (EGF).. EGF was significantly increased 12 months after both interventions. IL-6, IL-8 and MMP-9 were significantly increased only after corneal refractive therapy. The inflammatory response for the corneal refractive therapy patients was found to be associated with the degree of myopia corrected and the presence of corneal staining. Moreover, an increased level of MMP-9 and EGF was found to be associated with the presence of corneal-pigmented arc in the corneal refractive therapy group.. This research showed long-term increased tear levels of inflammatory markers in subjects wearing corneal refractive therapy lenses when compared with continuous wear of silicone-hydrogel lenses or no lens wear.

Topics: Adult; Biomarkers; Contact Lenses, Hydrophilic; Cross-Sectional Studies; Enzyme-Linked Immunosorbent Assay; Epidermal Growth Factor; Eye Proteins; Female; Humans; Interleukin-6; Interleukin-8; Male; Matrix Metalloproteinase 9; Myopia; Prospective Studies; Tears; Time Factors; Wound Healing

Topics: Contact Lenses, Hydrophilic; Epidermal Growth Factor; Female; Humans; Interleukin-6; Interleukin-8; Male; Matrix Metalloproteinase 9; Myopia; Tears

Topics: Contact Lenses, Hydrophilic; Epidermal Growth Factor; Female; Humans; Interleukin-6; Interleukin-8; Male; Matrix Metalloproteinase 9; Myopia; Tears