interleukin-8 and Meningioma

To compare gene expression profiles between atypical meningiomas and normal meninges from the National Center of Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database to identify key genes and pathways.. The microarray datasets GSE43290 and GSE16581 were downloaded from Gene Expression Omnibus (GEO) database to explore the key genes involved in meningioma formation and reocurrence. Additionally, the relationship between the key genes identified and clinical factors, including age, survival, and recurrence, was assessed. To investigate the the biological functions of the key genes, the gene ontology (GO) and Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways enrichment analyses were performed. Finally, the differences in immune infiltration between atypical meningiomas and healthy tissues were assessed.. A total of 286 hub genes were identified, which were enriched in leukocyte migration, collagen-containing extracellular matrix, signaling receptor activator activity, and receptor-ligand activity. The differentially expressed genes(DEGs) were mainly enriched in the IL-17 signaling pathway and focal adhesion. Among these hub genes, two overlapping genes, including CXCL8 and CXCL2, were selected as key genes which were correlated with tumor survival and recurrence. These two genes were enriched in mediating chemokine and cytokine responses, especially neutrophil and granulocyte responses, and influenced the immune cells infiltrated in the tumor tissue, thereby, influencing prognosis.. The implications of this study excavated the key genes in atypical meningiomas which could help us understand the molecular mechanisms and provide the candidate therapeutic targets.

Topics: Chemokine CXCL2; Computational Biology; Gene Expression Profiling; Gene Ontology; Humans; Interleukin-8; Meningeal Neoplasms; Meningioma

Topics: Animals; Dog Diseases; Dogs; Female; Interleukin-6; Interleukin-8; Male; Meningeal Neoplasms; Meningioma; Neoplasm Grading

The interactions of bacterial pathogens with cells of the human leptomeninges are critical events in the progression of meningitis. An in vitro model based on the culture of human meningioma cells was used to investigate the interactions of the meningeal pathogens Escherichia coli K1, Haemophilus influenzae, Neisseria meningitidis and Streptococcus pneumoniae. A rank order of association with meningioma cells was observed, with N. meningitidis showing the highest levels of adherence, followed by E. coli, S. pneumoniae and H. influenzae. Neisseria meningitidis and H. influenzae did not invade meningioma cells or induce cell death, but induced a concentration-dependent secretion of inflammatory mediators. Neisseria meningitidis induced higher levels of IL-6, MCP-1, RANTES and GM-CSF than H. influenzae, but there was no significant difference in the levels of IL-8 induced by both pathogens. Streptococcus pneumoniae was also unable to invade meningioma cells, but low concentrations of bacteria failed to stimulate cytokine secretion. However, higher concentrations of pneumococci led to cell death. By contrast, only E. coli K1 invaded meningioma cells directly and induced rapid cell death before an inflammatory response could be induced. These data demonstrate that the interactions of different bacterial pathogens with human meningeal cells are distinct, and suggest that different intervention strategies may be needed in order to prevent the morbidity and mortality associated with bacterial meningitis.

Topics: Bacterial Adhesion; Cell Death; Cell Line, Tumor; Chemokine CCL2; Chemokine CCL5; Colony Count, Microbial; Cytokines; Cytoplasm; Escherichia coli; Granulocyte-Macrophage Colony-Stimulating Factor; Haemophilus influenzae; Humans; Interleukin-6; Interleukin-8; Meninges; Meningioma; Meningitis, Bacterial; Microscopy, Confocal; Microscopy, Electron; Neisseria meningitidis; Streptococcus pneumoniae