interleukin-8 and Hepatitis--Viral--Human

Topics: Cell Adhesion; Chronic Disease; Cytokines; Hepatitis; Hepatitis, Alcoholic; Hepatitis, Viral, Human; Humans; Inflammation Mediators; Interleukin-1; Interleukin-10; Interleukin-2; Interleukin-4; Interleukin-5; Interleukin-6; Interleukin-8; Liver; Liver Cirrhosis; Tumor Necrosis Factor-alpha

To observe the clinical efficacy of treating infant cytomegalovirus (CMV) hepatitis with ganciclovir and impact on cytokines.. 76 patients with CMV hepatitis infant were randomly divided into treatment group and control group. The patients of two groups were treated using conventional therapy, on the basis of conventional therapy the treatment group was treated using induction and maintenance treatment of ganciclovir. Before and after treatment the growth and decline in jaundice, liver function, side effects and IL-8 and IFN-γ levels of the patients were detected, clinical efficacy was evaluated.. The clinical total effective rate of the treatment group was 91.4%, it was significantly higher than that of the control group (71.4%), compared with the control group the difference was significant (P < 0.05). After treatment, the growth and decline of jaundice (TBIL and DBIL), liver enzymes (ALT and AST) and cytokines (IL-8 and IFN-γ) levels increased, compared with the pre-treatment and the control group the difference was significant (P < 0.05). The treatment group was no adverse reaction.. The induction and maintenance treatment of ganciclovir in treatment of infant CMV hepatitis can make the body to restore balance of specific cellular immunity, and can significantly improve the symptoms of jaundice, liver function and clinical efficay and it is worthy to be popularized.

Topics: Antiviral Agents; Cytokines; Cytomegalovirus Infections; Female; Ganciclovir; Hepatitis, Viral, Human; Humans; Infant; Interferon-gamma; Interleukin-8; Male

Dengue is the most common mosquito-borne flaviviral infection in the world today. Several factors contribute and act synergistically to cause severe infection. One of these is dysregulated host immunological mediators that cause transient pathophysiology during infection. These mediators act on the endothelium to increase vascular permeability, which leads to plasma leakage compromising hemodynamics and coagulopathy. We conducted a prospective study to explore the expression of pro- and anti-inflammatory cytokines and how they relate to clinical dengue manifestations, by assessing their dynamics through acute dengue infection in adults admitted to the Hospital for Tropical Diseases, Bangkok, Thailand. We performed cytokine analysis at three phases of infection for 96 hospitalized adults together with serotyping of confirmed dengue infection during the outbreaks of 2015 and 2016. The serum concentrations of seven cytokines (interleukin [IL]-2, IL-4, IL-6, IL-8, IL-10, tumor necrosis factor alpha, and interferon gamma) were measured in duplicate using a commercial kit (Bio-Plex Human Cytokine Assay). In this study, the cytokine profile was suggestive of a T-helper 2 response. Most patients had secondary infection, and the levels of viremia were higher in patients with plasma leakage than those without plasma leakage. In addition, we observed that bleeding and hepatitis were associated with significantly higher levels of IL-8 during the early phases of infection. Furthermore, IL-6 levels in the early phase of infection were also elevated in bleeding patients with plasma leakage. These results suggest that IL-6 and IL-8 may act in synergy to cause bleeding in patients with plasma leakage.

Topics: Adult; Cytokines; Dengue; Female; Hemorrhage; Hepatitis, Viral, Human; Humans; Interferon-gamma; Interleukin-10; Interleukin-4; Interleukin-6; Interleukin-8; Male; Prospective Studies; Severe Dengue; Tumor Necrosis Factor-alpha; Viral Load

Hepatocellular carcinoma (HCC) is one of the most aggressive cancers worldwide. In Egypt, the disease is usually detected in an advanced stage at which no treatment may be effective including surgery. Early detection of the disease is thus an important goal allowing the patient to be treated before the enlargement of the tumor or its metastasis to distant organs. Tumor markers are serological agents which serum level may be useful in predicting the presence of the tumor at early stages. Alpha fetoprotein (AFP) which is the golden marker for HCC is of low sensitivity, therefore, additional markers such as alpha-L-fucosidase (AFU), transforming growth factors alpha and beta (TGF-α and TGF-β) and interleukin-8 (IL-8) are suggested to be simultaneously evaluated in order to enhance the detection of HCC. A total of 96 patients with different liver diseases such as HCC, hepatitis C virus (HCV), hepatitis B virus (HBV) and cirrhotic patients are included in this study. Sixteen healthy volunteers are used as a control group. In patients with HCC each of AFP, AFU, TGF-α and TGF-β recorded significantly higher levels than the other patient groups and controls. HCC patients recorded significantly lower level of IL-8 compared to the other patient groups but significantly higher than the control. For AFP, AFU, TGF-α, TGF-β and IL-8, at the optimal cut-off values (obtained from the receiver operating characteristic (ROC) curves), the calculated sensitivities are 46%, 72.97%, 67.56%, 54.05% and 83.8%, respectively. The simultaneous evaluation using all of the suggested markers resulted in increasing the sensitivity up to 100%. It thus recommended that, if patients with cirrhosis, as high risk patients, are subjected to regular examination using these markers in addition to AFP, HCC may be detected by 100% sensitivity in an early stage and as a consequence an effective treatment can be achieved.

Topics: Adult; Aged; alpha-Fetoproteins; alpha-L-Fucosidase; Biomarkers, Tumor; Carcinoma, Hepatocellular; Case-Control Studies; Early Detection of Cancer; Egypt; Female; Fibrosis; Hepatitis, Viral, Human; Humans; Interleukin-8; Liver Neoplasms; Male; Middle Aged; ROC Curve; Transforming Growth Factor alpha; Transforming Growth Factor beta

Topics: Adult; Female; Hepatitis, Viral, Human; Humans; Inflammation Mediators; Interleukin-1; Interleukin-6; Interleukin-8; Male; Systemic Inflammatory Response Syndrome; Tumor Necrosis Factor-alpha

To explore the relation between the pathogenesis and IL-8 level in the chronic hepatic disease and primary hepatocellular carcinoma.. 5ml venous blood was with drawn from 80 hospitaliged patients with different types of hepatic diseases and 14 healthy people. The serum was separated from the blood and then kept at -40 degrees Centigrade, and finally detected for IL-8 by ELISA.. There was an obvious difference among the IL-8 level in the serum from different types of hepatic disease patients. The IL-8 level was 75.80 microg/L 33.39 microg/L in chronic virus hepatitis and it was 89.54 microg/L 13.24 microg/L for primary hepatoma patients (t=10.48 and 4.01, respectively, P<0.01, as compared with control group).. There is a close relation between the level of IL-8 in serum and the state of illness. For patients with chronic hepatic diseases and primary hepatocellular carcinoma, the higher the IL-8 level is, the more serious the patients' condition, the worse the prognosis, and the higher the death rate would be.

Topics: Adult; Carcinoma, Hepatocellular; Chronic Disease; Enzyme-Linked Immunosorbent Assay; Female; Hepatitis; Hepatitis, Viral, Human; Humans; Interleukin-8; Liver Diseases; Liver Neoplasms; Male; Middle Aged

Topics: Adult; Aged; Cytokines; Female; Hepatitis, Viral, Human; Humans; Interleukin-1; Interleukin-8; Liver Cirrhosis; Male; Middle Aged; Nitric Oxide; Tumor Necrosis Factor-alpha

To investigate the cellular immunological changes in children with viral hepatitis, interleukin 6 (IL-6), interleukin 8 (IL-8), interferon alpha (IFN alpha), and tumor necrosis factor alpha (TNF alpha) in supernatant of cultured peripheral blood mononuclear cells (PBMCs) of 49 children with hepatitis A, B or C were measured. The levels of IL-6, IL-8, TNF alpha in PBMCs of the 3 viral hepatitis groups were increased and the level of IFN alpha decreased as compared with those of normal control group. But there were no significant differences among the 3 viral hepatitis groups. It was concluded that cellular immunological disorders were related to the onset and the induced damage of the viral hepatitis in children.

Topics: Adolescent; Child; Child, Preschool; Female; Hepatitis A; Hepatitis B, Chronic; Hepatitis C; Hepatitis, Viral, Human; Humans; Infant; Interleukin-6; Interleukin-8; Leukocytes, Mononuclear; Male; Tumor Necrosis Factor-alpha

Neutrophil chemotactic and functional defects occur in beta-thalassemia and in patients after bone marrow transplantation (BMT). Interleukin-8 (IL-8) is a novel chemotactic and activating peptide for neutrophils and can be detected in the circulation. IL-8 serum concentrations were evaluated in 30 beta-thalassemic patients before and after BMT. Serial samples from 16 patients were also studied. Fourteen sera from healthy children, 43 patients with chronic viral hepatitis, 16 patients on chronic transfusion treatment for various hematologic disorders, and 28 healthy adults were studied as controls. IL-8 was evaluated by an enzyme-linked immunosorbent assay. Patients with beta-thalassemia had higher IL-8 concentrations than did normal controls, patients with liver disease, and patients on chronic transfusion. beta-Thalassemic patients with severe liver siderosis and fibrosis had the highest IL-8 concentrations. After BMT in patients with successful engraftment, IL-8 concentrations decreased significantly. In contrast, in patients with acute graft-versus-host disease (GVHD), IL-8 concentrations were not statistically different from the concentrations found before BMT and were higher than in patients with no complications and patients with graft rejection. IL-8 may play a part in the immune dysregulation that occurs in beta-thalassemia and may be involved in the immune mechanisms leading to GVHD.

Topics: Adolescent; Adult; beta-Thalassemia; Biomarkers; Biopsy; Blood Transfusion; Bone Marrow Transplantation; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Female; Graft vs Host Disease; Hepatitis, Viral, Human; Humans; Interleukin-8; Liver; Male; Reference Values; Time Factors