interleukin-8 and Hand-Dermatoses

No biomarkers have been identified that can classify subtypes of hand eczema (HE). Although skin biopsies represent the gold standard for investigations of the skin, the invasive technique is not favorable when investigating skin from sensitive areas. Recent advances in the use of skin-tape strips for molecular investigations enable noninvasive investigations of HE.. By using whole transcriptome sequencing (WTS), the molecular profile of HE according to different localizations on the hands, etiologies, and clinical/morphological subtypes was investigated.. Thirty adult, Danish HE patients, 12 with and 18 without concurrent atopic dermatitis (AD), as well as 16 controls were included. Tape strip samples were collected from lesional, nonlesional, and healthy skin. Total RNA was extracted and WTS was performed.. The largest molecular difference of HE patients with and without AD was found in nonlesional skin areas and included a downregulation of CXCL8 for HE patients without AD. Differences between allergic and irritant contact dermatitis included epidermal biomarkers such as EPHA1.. Skin tape strip samples could be used to assess the gene expression profile of HE on different localizations of the hands. The skin tape strip method identified new molecular markers that showed promising result for the identification of HE subtypes.

Topics: Adult; Aged; Biomarkers; Dermatitis, Allergic Contact; Dermatitis, Atopic; Dermatitis, Irritant; Diagnosis, Differential; Down-Regulation; Exome Sequencing; Female; Hand Dermatoses; Humans; Interleukin-8; Male; Middle Aged; Receptor, EphA1; Skin; Specimen Handling; Surgical Tape; Transcriptome

Cytokines play an important role in skin inflammation.. We determined whether polymorphisms in cytokine genes contribute to the occurrence of occupational chronic irritant contact dermatitis (CICD).. In a case-control study, 9 polymorphisms in the genes coding for interleukin (IL)-1 alpha, IL-1 beta, IL-8, IL-10, and tumour necrosis factor (TNF)-alpha were determined in 197 patients with CICD. 217 apprentices in vocational training for high-risk occupations for CICD served as controls.. For all polymorphisms, no differences in genotype distributions were found between patients and controls. However, in patients with self-reported low levels of wet work and irritant exposure, more TNFA -308 variant genotypes (G/A and A/A) were present compared with those exposed to higher levels or controls, which indicates a TNFA-induced increase of susceptibility. In patients with TNFA -308 variant genotypes, the prevalence of flexural eczema was higher (48% and 57%) compared with that in patients presented with wild-type genotype (30%). Regarding IL1A -889, prevalence of symptoms of dermatitis was lower in apprentices with T/T or C/T genotype (32% and 36%) compared with wild-type genotype (54%, C/C). This indicates a protective effect of these variant alleles in acquiring hand dermatitis.. This study provides evidence that some genetic variations alter susceptibility to (chronic) dermatitis. Knowledge of the impact of genetic differences on the risk of CICD is essential in predictive testing of individuals at risk.

Topics: Adolescent; Adult; Case-Control Studies; Chronic Disease; Dermatitis, Atopic; Dermatitis, Irritant; Dermatitis, Occupational; DNA; Female; Gene Frequency; Genetic Predisposition to Disease; Genotype; Hand Dermatoses; Humans; Interleukin-10; Interleukin-1alpha; Interleukin-1beta; Interleukin-8; Logistic Models; Male; Middle Aged; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Tumor Necrosis Factor-alpha