interleukin-8 and Gallstones

Chile has high incidence rates of gallbladder cancer globally, particularly among Amerindian women, who also have a high prevalence of gallstones. We examined differences in inflammatory biomarkers between Mapuche and non-Mapuche women from the Chile Biliary Longitudinal Study, a cohort of women with ultrasound-detected gallstones. We randomly selected 200 Mapuche women frequency matched to non-Mapuche women on age and statin use Inflammatory biomarkers were analyzed using a multiplex assay and linear regression to assess associations of a priori markers (CCL20, CXCL10, IL-6, and IL-8) with ethnicity. Novel biomarkers were analyzed using exploratory factor analysis (EFA) and sufficient dimension reduction (SDR) to identify correlated marker groups, followed by linear regression to examine their association with ethnicity. The mean values of IL-8 were higher in Mapuche than non-Mapuche women (P = 0.04), while CCL20, CXCL10, and IL-6 did not differ significantly by ethnicity. EFA revealed two marker groups associated with ethnicity (P = 0.03 and P < 0.001). SDR analysis confirmed correlation between the biomarkers and ethnicity. We found higher IL-8 levels among Mapuche than non-Mapuche women. Novel inflammatory biomarkers were correlated with ethnicity and should be studied further for their role in gallbladder disease. These findings may elucidate underlying ethnic disparities in gallstones and carcinogenesis among Amerindians.

Topics: Aged; Carcinogenesis; Chemokine CCL20; Chemokine CXCL10; Chile; Ethnicity; Female; Gallbladder; Gallbladder Neoplasms; Gallstones; Genetic Association Studies; Genetic Predisposition to Disease; Humans; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Indians, South American; Inflammation; Interleukin-6; Interleukin-8; Longitudinal Studies; Middle Aged; Ultrasonography

Among the factors contributing to the gallstones formation an important role belongs to the inflammation. The goal of our research - determination alterations of the redox balance and cytokines (IL-1α, IL-6, IL-8, TNF-α) content in the blood postmenopausal women with gallstone disease. 58 menopausal women with gallstone disease, who had been admitted to the LTD "1-st Clinic" (Tbilisi, Georgia) during 2009-2011 were studied. Gallstone disease was proved by Ultrasonography method. The control group consisted of 25 menopause aged women without gallstone disease. The Local Ethics Committee approved the protocol, and informed consent was obtained from all participants or their surrogates. Patients was conducted for blood redox status (EPR signals of superoxide (O2-) and lipoperoxide (LOO.) radicals) and citokins (IL-1α, IL-6, IL-8, TNF-α) content. It was revealed increase production of oxygen and lipoperoxide free radicals, macrophage inflammatory cytokines (IL-6, IL-1α, TNF-α) in blood of menopausal women with gallstone disease. It was concluded that macrophages play a dominant role in the inflammatory and oxidative response during gallbladder stones disease in postmenopausal women.

Topics: Gallstones; Humans; Inflammation; Interleukin-1alpha; Interleukin-6; Interleukin-8; Lipid Peroxidation; Macrophages; Oxidative Stress; Tumor Necrosis Factor-alpha

Genetic variants in inflammation-related genes have been associated with biliary stones and biliary tract cancers in previous studies.. To follow-up on these findings, we examined 35 single nucleotide polymorphism (SNPs) in 5 genes related to inflammation (IL8, NFKBIL, RNASEL, TNF, and VEGFA) in 456 participants with incident biliary tract cancer cases (262 gallbladder, 141 extrahepatic bile duct, 53 ampulla of Vater), 982 participants with biliary stones, and 860 healthy controls in a population-based case-control study in Shanghai, China.. Suggestive associations were observed for SNPs in VEGFA with biliary stones, IL8 with gallbladder and ampulla of Vater cancers, and RNASEL with ampulla of Vater cancer (false discovery rate≤0.2).. These findings provide additional support for the role of inflammation in biliary stones and biliary tract cancer risk and need further validation.

Topics: Aged; Biliary Tract Neoplasms; Case-Control Studies; China; Endoribonucleases; Female; Gallstones; Genetic Predisposition to Disease; Genotype; Humans; Inflammation; Interleukin-8; Male; Middle Aged; Polymorphism, Single Nucleotide; Vascular Endothelial Growth Factor A

If differences of inflammatory pathways in acute pancreatitis exist for various etiologies, selective and specific antiinflammatory and other modulatory treatment regimens might be indicated. Circulating levels of prominent proinflammatory cytokines IL-6, 8, 18, and TNF-alpha were measured in patients having their first attack of either alcohol- or gallstone-induced acute pancreatitis.. Seventy-five consecutive patients were prospectively included over a 15-month period, sixty of them being either alcohol- or gallstone-induced. All patients were treated according to a standardized algorithm. Blood samples were obtained immediately on admission and, again, at days 1, 2, and 14.. A significant effect of the etiology on the levels of IL-8 in the alcohol group as compared to the gallstone group (P=0.003) was found. No etiologic differences were observed for IL-6, IL-18, TNF-alpha, or CRP. Furthermore, no significant differences, either regarding the need for treatment at the intensive care unit or of 30-day mortality, were found.. The present study confirms previous findings and supports the hypothesis that, except for IL-8, the biochemical profile and clinical outcome is independent of the underlying etiology. Revealing the complex spatial and temporal profile of proinflammatory cytokine expression in acute pancreatitis is necessary and important for the development of a more targeted rational therapy.

Topics: Adult; Aged; Aged, 80 and over; Biomarkers; C-Reactive Protein; Cytokines; Female; Gallstones; Humans; Interleukin-18; Interleukin-6; Interleukin-8; Male; Middle Aged; Pancreatitis; Pancreatitis, Alcoholic; Prospective Studies; Severity of Illness Index; Tumor Necrosis Factor-alpha; Young Adult

Non-obese gallstone patients exhibit a diminished expression of apical sodium-dependent bile acid transporter (ASBT) in terminal ileum. Crohn's ileitis demonstrates a significant downregulation of this transporter.. To test whether subclinical ileal inflammation contributes to gallstone disease.. Biopsies from terminal ileum of female subjects with gallstone disease (n = 7), active Crohn's disease (n = 17) and controls (n = 22) were investigated. mRNA expression of ASBT, tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, IL-8, c-jun and c-fos was measured. c-jun and c-fos protein levels were determined and hematoxylin and eosin staining was applied for ileal histology.. ASBT expression was comparably low both in gallstone (47% of controls, p = 0.0093) and Crohn's disease (42% of controls, p = 0.0008). In gallstone disease there was a non-significant trend towards elevated TNF-alpha and IL-1beta, but all cytokines were increased in active Crohn's disease. c-jun and c-fos were slightly diminished in patients with gallstones. Neither cytokines nor transcription factors correlated significantly with ASBT. The gallstone-associated ileal biopsies exhibited no histological inflammation.. Although the expression of ASBT was similarly diminished in both gallstone and Crohn's disease, subclinical ileal inflammation does not appear to be relevant in gallstone patients. The mechanisms of transcriptional repression of ASBT in both diseases are apparently different.

Topics: Adult; Aged; Case-Control Studies; Crohn Disease; Female; Gallstones; Humans; Ileitis; Ileum; Inflammation Mediators; Interleukin-1beta; Interleukin-8; Middle Aged; Organic Anion Transporters, Sodium-Dependent; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-jun; RNA, Messenger; Symporters; Tumor Necrosis Factor-alpha