interleukin-8 and Ependymoma

To determine the time course and clinical significance of cytokines and peptide growth factors in pediatric patients with ependymoma treated with postoperative radiotherapy (RT).. We measured 15 cytokines and growth factors (fibroblast growth factor, epidermal growth factor, vascular endothelial growth factor [VEGF], interleukin [IL]-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, interferon-gamma, tumor necrosis factor-alpha, granulocyte-macrophage colony-stimulating factor, monocyte chemoattractant protein-1, and macrophage inflammatory protein-alpha) from 30 patients before RT and 2 and 24 h, weekly for 6 weeks, and at 3, 6, 9, and 12 months after the initiation of RT. Two longitudinal models for the trend of log-transformed measurements were fitted, one during treatment and one through 12 months.. During RT, log IL-8 declined at a rate of -0.10389/wk (p = 0.0068). The rate of decline was greater (p = 0.028) for patients with an infratentorial tumor location. The decline in IL-8 after RT was significant when stratified by infratentorial tumor location (p = 0.0345) and more than one surgical procedure (p = 0.0272). During RT, the decline in log VEGF was significant when stratified by the presence of a ventriculoperitoneal shunt. After RT, the log VEGF declined significantly at a rate of -0.06207/mo. The decline was significant for males (p = 0.0222), supratentorial tumors (p = 0.0158), one surgical procedure (p = 0.0222), no ventriculoperitoneal shunt (p = 0.0005), and the absence of treatment failure (p = 0.0028).. The pro-inflammatory cytokine IL-8 declined significantly during RT and the decline differed according to tumor location. The angiogenesis factor VEGF declined significantly during the 12 months after RT. The decline was greater in males, those without a ventriculoperitoneal shunt, and in those with favorable disease factors, including one surgical procedure, supratentorial tumor location, and tumor control.

Topics: Adolescent; Analysis of Variance; Brain Neoplasms; Child; Child, Preschool; Cytokines; Ependymoma; Female; Humans; Infant; Intercellular Signaling Peptides and Proteins; Interleukin-8; Male; Neoplasm Proteins; Radiotherapy, Conformal; Time Factors; Vascular Endothelial Growth Factor A

To detect the expression and secretion of IL-8 in the ependymal tumor tissue and in primarily cultured cells, and to explore the relationship between IL-8 and ependymal tumor.. Expression of IL-8 in 50 ependymal tumor specimens was examined by immunohistochemistry. The ependymal tumor tissue obtained during operation was primarily cultured and IL-8 secreted by cultured cells were examined by clouble antibody 5 and which ELISA. The relations of expression of IL-8 to ependymal tumors of different histological degree, and invasiveness was analyzed statistically.. All the ependymal tumor tissue expressed IL-8, but the number of IL-8 positive cells in the anaplastic and malignant ependymoma was evidently larger than that in ependymoma (P < 0.01). All the cells cultured from the ependymal tumor secreted IL-8, but the liquid supernatant of anaplastic and malignant ependymoma cells contained more IL-8 than that of ependymoma cells (P < 0.01). The expression and secretion of IL-8 had no close relation to age and sex of patient.. The expression and secretion of IL-8 may be involved in the progression of ependymal tumor, so IL-8 can be used as an indicator to detect the malignant degree of ependymal tumor.

Topics: Adolescent; Adult; Brain Neoplasms; Child; Ependymoma; Female; Humans; Immunohistochemistry; Interleukin-8; Male; Middle Aged