interleukin-8 and Constipation

Constipation is currently one of the most common gastrointestinal disorders, and its causes are diverse. Multi-strain probiotics are often considered a more effective treatment than single-strain probiotics. In this study, a constipation model was constructed using loperamide hydrochloride to evaluate the ability of a multi-strain probiotic combination of four different ratios of

Topics: Animals; Bifidobacterium; Constipation; Gastrointestinal Diseases; Interleukin-6; Interleukin-8; Lactobacillus; Loperamide; Mice; Probiotics

To determine whether oxidative stress and inflammation are associated with constipation by examining the expression of the main producers of reactive oxygen species, nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, and pro-inflammatory cytokines in the colon of patients with chronic functional constipation. The colonic biopsies were collected from 32 patients with chronic functional constipation and 30 healthy subjects who underwent colonoscopy. Colonic mucosal histology was observed. Interleukin (IL)-1β, IL-6, IL-8 messenger RNA (mRNA), and 4 members of NADPH oxidase (NOX1, NOX2, DUOX2, and NOX4) protein and mRNA were assessed by immunohistochemistry, western blotting, and reverse transcription polymerase chain reaction. The tissues from both patients and healthy subjects showed normal histological structure without increase of inflammatory cells. NOX1 protein and mRNA levels were significantly increased compared to controls (P < .05). DUOX2 protein, but not mRNA, was increased by 2-fold compared to controls (P < .05). The levels of NOX2 and NOX4 protein and mRNA demonstrated no significant difference between patients and control subjects. The levels of IL-1β and IL-6 mRNA were significantly higher in constipation patients (P < .05), while IL-8 mRNA level was no different between the 2 groups. NADPH oxidase and pro-inflammatory cytokine might be involved in the pathogeneses of chronic functional constipation.

Topics: Colon; Constipation; Cytokines; Dual Oxidases; Humans; Interleukin-6; Interleukin-8; Mucous Membrane; NADPH Oxidase 1; NADPH Oxidase 4; NADPH Oxidases; Reactive Oxygen Species; RNA, Messenger

We used a whole-gut perfusion technique to study subclinical gut inflammation in children with cystic fibrosis (18 elective tests, three lavages to treat distal intestinal obstruction syndrome); and in 12 control children with constipation or pre-colonoscopy. We assayed for haemoglobin, IgG, albumin, alpha-1-antitrypsin, granulocyte elastase, interleukin-1 beta (IL-1 beta) and IL-8 concentrations in whole-gut lavage fluid. Results for two children with distal intestinal obstruction syndrome, the only children in the series taking Nutrizym 22, were strikingly abnormal. This new test has revealed subclinical gut mucosal inflammation in a minority of CF children, for which distal intestinal obstruction syndrome, Nutrizym 22 treatment, or both, may be risk factors.

Topics: Adolescent; Albumins; alpha 1-Antitrypsin; Amylases; Bromelains; Case-Control Studies; Child; Child, Preschool; Colitis; Colonoscopy; Constipation; Cystic Fibrosis; Drug Combinations; Female; Gastrointestinal Agents; Hemoglobins; Humans; Immunoglobulin G; Interleukin-1; Interleukin-8; Intestinal Obstruction; Leukocyte Elastase; Lipase; Male; Pancreatic Elastase; Pancreatic Extracts; Pancreatin; Pancrelipase; Risk Factors; Syndrome; Therapeutic Irrigation; Trypsin