interleukin-8 and Back-Pain

The mRNA expressions of cytokines and chemokines were assessed in herniated lumbar disc specimens.. To investigate whether the mRNAs of interleukin (IL)-1alpha, tumor necrosis factor (TNF)-alpha, RANTES, IL-8, IL-10, and transforming growth factor (TGF)-beta are expressed in surgically obtained herniated disc specimens; and to discover which of them are the predominant cytokines associated with the clinical symptoms and signs, and whether any differences in the mRNA expression exist depending on the different types of disc herniations.. It has been postulated that cytokines are involved in causing radicular leg pain in lumbar disc herniations. Although a few studies have been done on lumbar disc herniations concerning IL-1alpha and TNF-alpha, almost none has been carried out in the cases of the other of cytokines and chemokines.. Using a reverse transcription-polymerase chain reaction, mRNA expressions of cytokines and chemokines were investigated in herniated disc specimens. The straight leg raising test, development of radicular pain by back extension, symptom duration, pain intensity using a visual analogue scale, and herniation types were described.. The mRNAs of IL-8, TNF-alpha, IL-1alpha, RANTES, and IL-10 were expressed in 16 (70%), 15 (65%), 9 (39%), 4 (17%), and 2 (9%) of the 23 herniated disc specimens, respectively. The mRNA of TGF-beta was expressed in 5 of 10 specimens (50%). IL-8 mRNA expression was associated with the development of radicular pain by back extension and short symptom duration (average 3.8 weeks). The mRNAs of IL-1alpha were expressed more frequently in transligamentous extensions than in subligamentous extensions, but the expression was weak.. Interleukin-8 appears to be associated with development of radicular pain by back extension and to be activated on acute or subacute disc herniations. IL-8 seems to participate in the pathomechanism of nerve root inflammation in lumbar disc herniations, which implies that it may be considered a target for therapeutic intervention.

Topics: Adult; Aged; Back Pain; Chemokine CCL5; Chemokines; Cytokines; Female; Humans; Interleukin-1; Interleukin-10; Interleukin-8; Intervertebral Disc Displacement; Lumbosacral Region; Male; Middle Aged; Predictive Value of Tests; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Transforming Growth Factor beta; Tumor Necrosis Factor-alpha

To examine the link between cytokines in intervertebral disc (IVD) tissues and axial back pain.. In vitro study with human IVD cells cultured from cadaveric donors and annulus fibrosus (AF) tissues from patients.. Cultured nucleus pulposus (NP) and AF cells were stimulated with interleukin (IL)-1β. IL-8 and IL-7 gene expression was analyzed using real-time polymerase chain reaction. IL-8 protein was quantified by enzyme-linked immunosorbent assay. After IL-1β stimulation, IL-8 gene expression increased 26,541 fold in NP cells and 22,429 fold in AF cells, whereas protein released by the NP and AF cells increased 2,389- and 1,784-fold, respectively. IL-7 gene expression increased 3.3-fold in NP cells (P < 0.05).Cytokine profiles in AF tissues collected from patients undergoing surgery for back pain (painful group) or scoliosis (controls) were compared by cytokine array. IL-8 protein in the AF tissues from patients with back pain was 1.81-fold of that in controls. IL-7 and IL-10 in AF tissues from the painful group were 6.87 and 4.63 times greater than the corresponding values in controls, respectively (P < 0.05).. Inflammatory mediators found in AF tissues from patients with discogenic back pain are likely produced by IVD cells and may play a key role in back pain.

Topics: Adult; Aged; Annulus Fibrosus; Back Pain; Enzyme-Linked Immunosorbent Assay; Female; Gene Expression; Humans; Interleukin-10; Interleukin-7; Interleukin-8; Interleukins; Intervertebral Disc; Male; Middle Aged; Nucleus Pulposus; Real-Time Polymerase Chain Reaction; Young Adult